دورية أكاديمية
Effects of Propofol on Several Membrane Characteristics of Cervical Cancer Cell Lines
| العنوان: | Effects of Propofol on Several Membrane Characteristics of Cervical Cancer Cell Lines |
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| المؤلفون: | Fan Zhang, Changsong Wang, Yinghua Cui, Shuping Li, Yuanfei Yao, Yanpeng Ci, Jinghe Wang, Wei Hou, Anqi Wu, Enyou Li |
| المصدر: | Cellular Physiology and Biochemistry, Vol 40, Iss 1-2, Pp 172-182 (2016) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Physiology LCC:Biochemistry |
| مصطلحات موضوعية: | AFM, Cytoskeleton, Migration, HeLa cell, Propofol, Physiology, QP1-981, Biochemistry, QD415-436 |
| الوصف: | Background: Although significant advances have been made toward understanding the molecular mechanisms underlying the effect of propofol on tumor cell metastasis, less is known regarding how cell membrane and cytoskeletal ultrastructure are affected in this process. Here, we investigated the relationship between cell morphology and cell size, which are features mainly defined by the cytoskeleton. Methods: To confirm the effects of propofol on the migratory ability of human cervical carcinoma cells, cell migration and invasion were examined through scratch wound healing and transwell membrane assays. Furthermore, HeLa cells cultivated with different concentrations of propofol were examined by confocal microscopy and atomic force microscopy (AFM), and the mean optical density and migration ability of these cells were also assessed. In addition, cell membrane morphology was inspected using AFM. Results: The results of the wound healing and transwell membrane assays indicated that propofol decreases the migratory ability of cervical carcinoma cells compared to control cells. A comparative analysis of the test results revealed that short-term (3 h) exposure to propofol induced marked changes in cell membrane microstructure and in the cytoskeleton in a dose-dependent manner. These morphological changes in the cell membrane were accompanied by cytoskeleton (F-actin) derangement. The present findings demonstrate a close relationship between changes in cell membrane ultrastructure and cytoskeletal alterations (F-actin) in propofol-treated HeLa cells. AFM scanning analysis showed that cell membrane ultrastructure was significantly changed, including a clear reduction in membrane roughness. Conclusion: The influence of propofol on the HeLa cell cytoskeleton can be directly reflected by changes in cellular morphology, as assessed by AFM. Moreover, the use of AFM is a good method for investigating propofol-mediated changes within cytoskeletal ultrastructure. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1015-8987 1421-9778 |
| Relation: | http://www.karger.com/Article/FullText/452535; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000452535 |
| URL الوصول: | https://doaj.org/article/a78ffa3e4c5a43428cbaef0e06f88e8f |
| رقم الأكسشن: | edsdoj.78ffa3e4c5a43428cbaef0e06f88e8f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10158987 14219778 |
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| DOI: | 10.1159/000452535 |