دورية أكاديمية
Mitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells
| العنوان: | Mitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells |
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| المؤلفون: | Jae-Eun Song, Tiago C. Alves, Bernardo Stutz, Matija Šestan-Peša, Nicole Kilian, Sungho Jin, Sabrina Diano, Richard G. Kibbey, Tamas L. Horvath |
| المصدر: | Metabolites, Vol 11, Iss 5, p 322 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | mitochondrial dynamics, fatty acid oxidation, lipid homeostasis, Microbiology, QR1-502 |
| الوصف: | In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 11050322 2218-1989 79220711 |
| Relation: | https://www.mdpi.com/2218-1989/11/5/322; https://doaj.org/toc/2218-1989 |
| DOI: | 10.3390/metabo11050322 |
| URL الوصول: | https://doaj.org/article/79220711625c484daab929828974ea71 |
| رقم الأكسشن: | edsdoj.79220711625c484daab929828974ea71 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11050322 22181989 79220711 |
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| DOI: | 10.3390/metabo11050322 |