دورية أكاديمية
Inhibitory Effects of Isoquinoline Alkaloid Berberine on Ischemia-Induced Apoptosis via Activation of Phosphoinositide 3-Kinase/Protein Kinase B Signaling Pathway
| العنوان: | Inhibitory Effects of Isoquinoline Alkaloid Berberine on Ischemia-Induced Apoptosis via Activation of Phosphoinositide 3-Kinase/Protein Kinase B Signaling Pathway |
|---|---|
| المؤلفون: | Mia Kim, Mal Soon Shin, Jae Min Lee, Han Sam Cho, Chang Ju Kim, Young Joon Kim, Hey Ran Choi, Jung Won Jeon |
| المصدر: | International Neurourology Journal, Vol 18, Iss 3, Pp 115-125 (2014) |
| بيانات النشر: | Korean Continence Society, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Berberine, Brain ischemia, Short-term memory, Apoptosis, Phosphatidylinositol 3-kinases, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | PurposeBerberine is a type of isoquinoline alkaloid that has been used to treat various diseases. A neuroprotective effect of berberine against cerebral ischemia has been reported; however, the effects of berberine on apoptosis in relation to reactive astrogliosis and microglia activation under ischemic conditions have not yet been fully evaluated. In the present study, we investigated the effects of berberine on global ischemia-induced apoptosis, and focused on the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway in the hippocampus using gerbils.MethodsGerbils received berberine orally once a day for 14 consecutive days, starting one day after surgery. In this study, a step-down avoidance task was used to assess short-term memory. Furthermore, we employed the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to evaluate DNA fragmentation, immunohistochemistry to investigate glial fibriallary acidic protein, CD11b, and caspase-3, and western blot to assess PI3K, Akt, Bax, Bcl-2, and cytochrome c.ResultsOur results revealed that berberine treatment alleviated ischemia-induced short-term memory impairment. Treatment with berbeine also attenuated ischemia-induced apoptosis and inhibited reactive astrogliosis and microglia activation. Furthermore, berberine enhanced phospho-PI3K and phospho-Akt expression in the hippocampus of ischemic gerbils.ConclusionsBerberine exerted a neuroprotective effect against ischemic insult by inhibiting neuronal apoptosis via activation of the PI3K/Akt signaling pathway. The antiapoptotic effect of berberine was achieved through inhibition of reactive astrogliosis and microglia activation. Berberine may therefore serve as a therapeutic agent for stroke-induced neurourological problems. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2093-4777 2093-6931 |
| Relation: | http://www.einj.org/upload/pdf/inj-18-115.pdf; https://doaj.org/toc/2093-4777; https://doaj.org/toc/2093-6931 |
| DOI: | 10.5213/inj.2014.18.3.115 |
| URL الوصول: | https://doaj.org/article/792b58da15124c76bdf5ca6ccf456268 |
| رقم الأكسشن: | edsdoj.792b58da15124c76bdf5ca6ccf456268 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20934777 20936931 |
|---|---|
| DOI: | 10.5213/inj.2014.18.3.115 |