دورية أكاديمية
RAS2 Regulates Growth and Pathogenesis in Fusarium graminearum
| العنوان: | RAS2 Regulates Growth and Pathogenesis in Fusarium graminearum |
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| المؤلفون: | B. H. Bluhm, X. Zhao, J. E. Flaherty, J.-R. Xu, L. D. Dunkle |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 20, Iss 6, Pp 627-636 (2007) |
| بيانات النشر: | The American Phytopathological Society, 2007. |
| سنة النشر: | 2007 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | Gibberella zeae, head blight, Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | Fusarium graminearum is a ubiquitous pathogen of cereal crops, including wheat, barley, and maize. Diseases caused by F. graminearum are of particular concern because harvested grains frequently are contaminated with harmful mycotoxins such as deoxynivalenol (DON). In this study, we explored the role of Ras GTPases in pathogenesis. The genome of F. graminearum contains two putative Ras GTPase-encoding genes. The two genes (RAS1 and RAS2) showed different patterns of expression under different conditions of nutrient availability and in various mutant backgrounds. RAS2 was dispensable for survival but, when disrupted, caused a variety of morphological defects, including slower growth on solid media, delayed spore germination, and significant reductions in virulence on wheat heads and maize silks. Intracellular cAMP levels were not affected by deletion of RAS2 and exogenous treatment of the ras2 mutant with cAMP did not affect phenotypic abnormalities, thus indicating that RAS2 plays a minor or no role in cAMP signaling. However, phosphorylation of the mitogen-activated protein (MAP) kinase Gpmk1 and expression of a secreted lipase (FGL1) required for infection were reduced significantly in the ras2 mutant. Based on these observations, we hypothesize that RAS2 regulates growth and virulence in F. graminearum by regulating the Gpmk1 MAP kinase pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-20-6-0627 |
| URL الوصول: | https://doaj.org/article/795de226ef394d67a5260b9d1a7b9fd8 |
| رقم الأكسشن: | edsdoj.795de226ef394d67a5260b9d1a7b9fd8 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-20-6-0627 |