دورية أكاديمية
Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum
| العنوان: | Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum |
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| المؤلفون: | Monica Lopes Ferreira, Maria Alice Pimentel Falcão, Fernanda Miriane Bruni, Vidal Haddad, Elineide Eugênio Marques, Carla Simone Seibert, Carla Lima |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 9, p 8338 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | polyclonal antiserum, neutralizing antibody, fish venom, toxic effects, Thalassophryne nattereri, Scorpaena plumieri, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Envenomation by venomous fish, although not always fatal, is capable of causing damage to homeostasis by activating the inflammatory process, with the formation of edema, excruciating pain, necrosis that is difficult to heal, as well as hemodynamic and cardiorespiratory changes. Despite the wide variety of pharmacological treatments used to manage acute symptoms, none are effective in controlling envenomation. Knowing the essential role of neutralizing polyclonal antibodies in the treatment of envenoming for other species, such as snakes, this work aimed to produce a polyclonal antiserum in mice and test its ability to neutralize the main toxic effects induced by the venoms of the main venomous Brazilian fish. We found that the antiserum recognizes the main toxins present in the different venoms of Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr. Orbignyi, and Cathorops spixii and was effective in pre-incubation trials. In an independent test, the antiserum applied immediately to the topical application of T. nattereri, P. gr orbygnyi, and C. spixii venoms completely abolished the toxic effects on the microcirculation, preventing alterations such as arteriolar contraction, slowing of blood flow in postcapillary venules, venular stasis, myofibrillar hypercontraction, and increased leukocyte rolling and adherence. The edematogenic and nociceptive activities induced by these venoms were also neutralized by the immediate application of the antiserum. Importantly, the antiserum prevented the acute inflammatory response in the lungs induced by the S. plumieri venom. The success of antiserum containing neutralizing polyclonal antibodies in controlling the toxic effects induced by different venoms offers a new strategy for the treatment of fish envenomation in Brazil. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/9/8338; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms24098338 |
| URL الوصول: | https://doaj.org/article/7a0931808f92488f80c310460d497ecb |
| رقم الأكسشن: | edsdoj.7a0931808f92488f80c310460d497ecb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms24098338 |