دورية أكاديمية
Evaluation of Antifungal Selective Toxicity Using Candida glabrata ERG25 and Human SC4MOL Knock-In Strains
| العنوان: | Evaluation of Antifungal Selective Toxicity Using Candida glabrata ERG25 and Human SC4MOL Knock-In Strains |
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| المؤلفون: | Keiko Nakano, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Kaname Sasamoto, Masashi Yamaguchi, Hiroji Chibana |
| المصدر: | Journal of Fungi, Vol 9, Iss 10, p 1035 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | antimicrobial resistance (AMR), molecular target drug, specific inhibitor, drug resistant, C4-methyl sterol oxidase, methylsterol monooxygenase 1 (MSMO1), Biology (General), QH301-705.5 |
| الوصف: | With only four classes of antifungal drugs available for the treatment of invasive systemic fungal infections, the number of resistant fungi is increasing, highlighting the urgent need for novel antifungal drugs. Ergosterol, an essential component of cell membranes, and its synthetic pathway have been targeted for antifungal drug development. Sterol-C4-methyl monooxygenase (Erg25p), which is a greater essential target than that of existing drugs, represents a promising drug target. However, the development of antifungal drugs must consider potential side effects, emphasizing the importance of evaluating their selective toxicity against fungi. In this study, we knocked in ERG25 of Candida glabrata and its human ortholog, SC4MOL, in ERG25-deleted Saccharomyces cerevisiae. Utilizing these strains, we evaluated 1181-0519, an Erg25p inhibitor, that exhibited selective toxicity against the C. glabrata ERG25 knock-in strain. Furthermore, 1181-0519 demonstrated broad-spectrum antifungal activity against pathogenic Candida species, including Candida auris. The approach of utilizing a gene that is functionally conserved between yeast and humans and subsequently screening for molecular target drugs enables the identification of selective inhibitors for both species. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2309-608X 12647179 |
| Relation: | https://www.mdpi.com/2309-608X/9/10/1035; https://doaj.org/toc/2309-608X |
| DOI: | 10.3390/jof9101035 |
| URL الوصول: | https://doaj.org/article/7a5e3893c12647179910d44d1feccd5c |
| رقم الأكسشن: | edsdoj.7a5e3893c12647179910d44d1feccd5c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2309608X 12647179 |
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| DOI: | 10.3390/jof9101035 |