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PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice

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العنوان: PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice
المؤلفون: Yuecheng Jiang, Yanzhe Peng, Xia Yang, Jiali Yu, Fuxun Yu, Jing Yuan, Yan Zha
المصدر: PeerJ, Vol 11, p e15856 (2023)
بيانات النشر: PeerJ Inc., 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Biology (General)
مصطلحات موضوعية: PM2.5, Diabetes mellitus, Diabetic kidney disease, Ectopic lipid accumulation, SREBP-1, ATGL, Medicine, Biology (General), QH301-705.5
الوصف: Background Ambient fine particulate matter ≤ 2.5 µm (PM2.5) air pollution exposure has been identified as a global health threat, the epidemiological evidence suggests that PM2.5 increased the risk of chronic kidney disease (CKD) among the diabetes mellitus (DM) patients. Despite the growing body of research on PM2.5 exposure, there has been limited investigation into its impact on the kidneys and the underlying mechanisms. Past studies have demonstrated that PM2.5 exposure can lead to lipid metabolism disorder, which has been linked to the development and progression of diabetic kidney disease (DKD). Methods In this study, db/db mice were exposed to different dosage PM2.5 for 8 weeks. The effect of PM2.5 exposure was analysis by assessment of renal function, pathological staining, immunohistochemical (IHC), quantitative real-time PCR (qPCR) and liquid chromatography with tandem mass spectrometry (LC–MS/MS) based metabolomic analyses. Results The increasing of Oil Red staining area and adipose differentiation related protein (ADRP) expression detected by IHC staining indicated more ectopic lipid accumulation in kidney after PM2.5 exposure, and the increasing of SREBP-1 and the declining of ATGL detected by IHC staining and qPCR indicated the disorder of lipid synthesisandlipolysis in DKD mice kidney after PM2.5 exposure. The expressions of high mobility group nucleosome binding protein 1 (HMGN1) and kidney injury molecule 1 (KIM-1) that are associated with kidney damage increased in kidney after PM2.5 exposure. Correlation analysis indicated that there was a relationship between HMGN1-KIM-1 and lipid metabolic markers. In addition, kidneys of mice were analyzed using LC–MS/MS based metabolomic analyses. PM2.5 exposure altered metabolic profiles in the mice kidney, including 50 metabolites. In conclusion the results of this study show that PM2.5 exposure lead to abnormal renal function and further promotes renal injury by disturbance of renal lipid metabolism and alter metabolic profiles.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2167-8359
Relation: https://peerj.com/articles/15856.pdf; https://peerj.com/articles/15856/; https://doaj.org/toc/2167-8359
DOI: 10.7717/peerj.15856
URL الوصول: https://doaj.org/article/7a8beadeaf004621b27e07a32aaac0a4
رقم الأكسشن: edsdoj.7a8beadeaf004621b27e07a32aaac0a4
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21678359
DOI:10.7717/peerj.15856