دورية أكاديمية
Long-term alteration of intestinal microbiota in patients with ulcerative colitis by antibiotic combination therapy.
العنوان: | Long-term alteration of intestinal microbiota in patients with ulcerative colitis by antibiotic combination therapy. |
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المؤلفون: | Shigeo Koido, Toshifumi Ohkusa, Takayuki Kajiura, Junko Shinozaki, Manabu Suzuki, Keisuke Saito, Kazuki Takakura, Shintaro Tsukinaga, Shunichi Odahara, Toyokazu Yukawa, Jimi Mitobe, Mikio Kajihara, Kan Uchiyama, Hiroshi Arakawa, Hisao Tajiri |
المصدر: | PLoS ONE, Vol 9, Iss 1, p e86702 (2014) |
بيانات النشر: | Public Library of Science (PLoS), 2014. |
سنة النشر: | 2014 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Medicine, Science |
الوصف: | Previous work has demonstrated that intestinal bacteria, such as Fusobacterium varium (F. varium), contribute to the clinical activity in ulcerative colitis (UC); thus, an antibiotic combination therapy (amoxicillin, tetracycline, and metronidazole (ATM)) against F. varium can induce and maintain UC remission. Therefore, we investigated whether ATM therapy induces a long-term alteration of intestinal microbiota in patients with UC. Patients with UC were enrolled in a multicenter, randomized, double-blind, placebo-controlled study. Biopsy samples at the beginning of the trial and again at 3 months after treatment completion were randomly obtained from 20 patients. The terminal restriction fragment length polymorphism (T-RFLP) in mucosa-associated bacterial components was examined to assess the alteration of the intestinal microbiota. Profile changes of T-RFLP in mucosa-associated bacterial components were found in 10 of 12 patients in the treatment group and in none of 8 in the placebo group. Dice similarity coefficients using the unweighted pair group method with arithmetic averages (Dice-UPGMA) confirmed that the similarity of mucosal microbiota from the descending colon was significantly decreased after the ATM therapy, and this change was maintained for at least 3 months. Moreover, at 3 months after treatment completion, the F. varium/β-actin ratio, examined by real-time PCR using nested PCR products from biopsy samples, was reduced less than 40% in 8 of 12 treated patients, which was higher, but not significantly, than in 4 of 8 patients in the placebo group. Together, these results suggest that ATM therapy induces long-term alterations in the intestinal microbiota of patients with UC, which may be associated, at least in part, with clinical effects of the therapy. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1932-6203 |
Relation: | http://europepmc.org/articles/PMC3906066?pdf=render; https://doaj.org/toc/1932-6203 |
DOI: | 10.1371/journal.pone.0086702 |
URL الوصول: | https://doaj.org/article/7ad10cf65380461f8d66dcb7dc5062ba |
رقم الأكسشن: | edsdoj.7ad10cf65380461f8d66dcb7dc5062ba |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0086702 |