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Long-term alteration of intestinal microbiota in patients with ulcerative colitis by antibiotic combination therapy.

التفاصيل البيبلوغرافية
العنوان: Long-term alteration of intestinal microbiota in patients with ulcerative colitis by antibiotic combination therapy.
المؤلفون: Shigeo Koido, Toshifumi Ohkusa, Takayuki Kajiura, Junko Shinozaki, Manabu Suzuki, Keisuke Saito, Kazuki Takakura, Shintaro Tsukinaga, Shunichi Odahara, Toyokazu Yukawa, Jimi Mitobe, Mikio Kajihara, Kan Uchiyama, Hiroshi Arakawa, Hisao Tajiri
المصدر: PLoS ONE, Vol 9, Iss 1, p e86702 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Previous work has demonstrated that intestinal bacteria, such as Fusobacterium varium (F. varium), contribute to the clinical activity in ulcerative colitis (UC); thus, an antibiotic combination therapy (amoxicillin, tetracycline, and metronidazole (ATM)) against F. varium can induce and maintain UC remission. Therefore, we investigated whether ATM therapy induces a long-term alteration of intestinal microbiota in patients with UC. Patients with UC were enrolled in a multicenter, randomized, double-blind, placebo-controlled study. Biopsy samples at the beginning of the trial and again at 3 months after treatment completion were randomly obtained from 20 patients. The terminal restriction fragment length polymorphism (T-RFLP) in mucosa-associated bacterial components was examined to assess the alteration of the intestinal microbiota. Profile changes of T-RFLP in mucosa-associated bacterial components were found in 10 of 12 patients in the treatment group and in none of 8 in the placebo group. Dice similarity coefficients using the unweighted pair group method with arithmetic averages (Dice-UPGMA) confirmed that the similarity of mucosal microbiota from the descending colon was significantly decreased after the ATM therapy, and this change was maintained for at least 3 months. Moreover, at 3 months after treatment completion, the F. varium/β-actin ratio, examined by real-time PCR using nested PCR products from biopsy samples, was reduced less than 40% in 8 of 12 treated patients, which was higher, but not significantly, than in 4 of 8 patients in the placebo group. Together, these results suggest that ATM therapy induces long-term alterations in the intestinal microbiota of patients with UC, which may be associated, at least in part, with clinical effects of the therapy.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3906066?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0086702
URL الوصول: https://doaj.org/article/7ad10cf65380461f8d66dcb7dc5062ba
رقم الأكسشن: edsdoj.7ad10cf65380461f8d66dcb7dc5062ba
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0086702