دورية أكاديمية
Antigens Expressed by Breast Cancer Cells Undergoing EMT Stimulate Cytotoxic CD8+ T Cell Immunity
| العنوان: | Antigens Expressed by Breast Cancer Cells Undergoing EMT Stimulate Cytotoxic CD8+ T Cell Immunity |
|---|---|
| المؤلفون: | Faye A. Camp, Tonya M. Brunetti, Michelle M. Williams, Jessica L. Christenson, Varsha Sreekanth, James C. Costello, Zachary L. Z. Hay, Ross M. Kedl, Jennifer K. Richer, Jill E. Slansky |
| المصدر: | Cancers, Vol 14, Iss 18, p 4397 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | neojunction, neoantigen, intron retention, mIR-200c, epithelial-to-mesenchymal transition (EMT), CD8+ T cells, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Antigenic differences formed by alterations in gene expression and alternative splicing are predicted in breast cancer cells undergoing epithelial to mesenchymal transition (EMT) and the reverse plasticity known as MET. How these antigenic differences impact immune interactions and the degree to which they can be exploited to enhance immune responses against mesenchymal cells is not fully understood. We utilized a master microRNA regulator of EMT to alter mesenchymal-like EO771 mammary carcinoma cells to a more epithelial phenotype. A computational approach was used to identify neoantigens derived from the resultant differentially expressed somatic variants (SNV) and alternative splicing events (neojunctions). Using whole cell vaccines and peptide-based vaccines, we find superior cytotoxicity against the more-epithelial cells and explore the potential of neojunction-derived antigens to elicit T cell responses through experiments designed to validate the computationally predicted neoantigens. Overall, results identify EMT-associated splicing factors common to both mouse and human breast cancer cells as well as immunogenic SNV- and neojunction-derived neoantigens in mammary carcinoma cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 14184397 2072-6694 |
| Relation: | https://www.mdpi.com/2072-6694/14/18/4397; https://doaj.org/toc/2072-6694 |
| DOI: | 10.3390/cancers14184397 |
| URL الوصول: | https://doaj.org/article/7b708c1718cc43708f7240b577a154ea |
| رقم الأكسشن: | edsdoj.7b708c1718cc43708f7240b577a154ea |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14184397 20726694 |
|---|---|
| DOI: | 10.3390/cancers14184397 |