دورية أكاديمية
IL-37 overexpression promotes endometrial regenerative cell-mediated inhibition of cardiac allograft rejection
| العنوان: | IL-37 overexpression promotes endometrial regenerative cell-mediated inhibition of cardiac allograft rejection |
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| المؤلفون: | Hong Qin, Chenglu Sun, Yanglin Zhu, Yafei Qin, Shaohua Ren, Zhaobo Wang, Chuan Li, Xiang Li, Baoren Zhang, Jingpeng Hao, Guangming Li, Hongda Wang, Bo Shao, Jingyi Zhang, Hao Wang |
| المصدر: | Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-14 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Endometrial regenerative cells, Interleukin-37, Acute allograft rejection, Mice, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Endometrial regenerative cells (ERCs) play an important role in attenuation of acute allograft rejection, while their effects are limited. IL-37, a newly discovered immunoregulatory cytokine of the IL-1 family, can regulate both innate and adaptive immunity. Whether IL-37 overexpression can enhance the therapeutic effects of ERCs in inhibition of acute cardiac allograft rejection remains unknown and will be explored in this study. Methods C57BL/6 mice recipients receiving BALB/c mouse heterotopic heart allografts were randomly divided into the phosphate-buffered saline (untreated), ERC treated, negative lentiviral control ERC (NC-ERC) treated, and IL-37 overexpressing ERC (IL-37-ERC) treated groups. Graft pathological changes were assessed by H&E staining. The intra-graft cell infiltration and splenic immune cell populations were analyzed by immunohistochemistry and flow cytometry, respectively. The stimulatory property of recipient DCs was tested by an MLR assay. Furthermore, serum cytokine profiles of recipients were measured by ELISA assay. Results Mice treated with IL-37-ERCs achieved significantly prolonged allograft survival compared with the ERC-treated group. Compared with all the other control groups, IL-37-ERC-treated group showed mitigated inflammatory response, a significant increase in tolerogenic dendritic cells (Tol-DCs), regulatory T cells (Tregs) in the grafts and spleens, while a reduction of Th1 and Th17 cell population. Additionally, there was a significant upregulation of immunoregulatory IL-10, while a reduction of IFN-γ, IL-17A, IL-12 was detected in the sera of IL-37-ERC-treated recipients. Conclusion IL-37 overexpression can promote the therapeutic effects of ERCs to inhibit acute allograft rejection and further prolong graft survival. This study suggests that gene-modified ERCs overexpressing IL-37 may pave the way for novel therapeutic options in the field of transplantation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-022-02982-1 |
| URL الوصول: | https://doaj.org/article/d7b7e12c87614637b93d0202a03114a3 |
| رقم الأكسشن: | edsdoj.7b7e12c87614637b93d0202a03114a3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-022-02982-1 |