دورية أكاديمية
(1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2.
العنوان: | (1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2. |
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المؤلفون: | Jinki Yeom, Ji-Hyun Shin, Ji-Young Yang, Jungmin Kim, Geum-Sook Hwang |
المصدر: | PLoS ONE, Vol 8, Iss 3, p e57730 (2013) |
بيانات النشر: | Public Library of Science (PLoS), 2013. |
سنة النشر: | 2013 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Medicine, Science |
الوصف: | Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1932-6203 |
Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483923/pdf/?tool=EBI; https://doaj.org/toc/1932-6203 |
DOI: | 10.1371/journal.pone.0057730 |
URL الوصول: | https://doaj.org/article/7bb265f02d9448249518bec99cfa4c43 |
رقم الأكسشن: | edsdoj.7bb265f02d9448249518bec99cfa4c43 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0057730 |