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Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey

التفاصيل البيبلوغرافية
العنوان: Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey
المؤلفون: Aytekin Cikman, Merve Aydin, Baris Gulhan, Faruk Karakecili, Muhammet G. Kurtoglu, Serife Yuksekkaya, Mehmet Parlak, Bilge S. Gultepe, Aysegul C. Cicek, Fulya B. Bilman, Ihsan H. Ciftci, Murat Kara, Selahattin Atmaca, Tuncer Ozekinci
المصدر: Journal of Infection and Public Health, Vol 12, Iss 4, Pp 528-533 (2019)
بيانات النشر: Elsevier, 2019.
سنة النشر: 2019
المجموعة: LCC:Infectious and parasitic diseases
LCC:Public aspects of medicine
مصطلحات موضوعية: Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270
الوصف: Background: mecA is a predefined gene causing methicillin resistance in Staphylococcus aureus (S. aureus) isolates; however, it has been shown that some methicillin-resistant S. aureus (MRSA) strains do not carry this gene. Recently, in isolates found to be MRSA-positive but mecA-negative, a new resistance gene called mecC, which is a homolog of mecA, has been reported. This study aimed to investigate the mecC and mecA genes in MRSA strains isolated from different geographic regions in Turkey. Methods: The sample of the study consisted of 494 MRSA strains isolated from seven geographical regions in Turkey between 2013 and 2016. The strains were obtained from 17 centers, comprising 13 university hospitals, three education and research hospitals, and one state hospital. Methicillin resistance in S. aureus strains was determined using the agar disk diffusion method with a cefoxitin disk and the agar dilution method with oxacillin. The mecC and mecA genes in MRSA strains was investigated by Polymerase Chain Reaction (PCR). Results: Of the MRSA strains investigated, 47.9% were isolated from intensive care units. Concerning sample type, 36.7% were detected in the respiratory tract (tracheal aspirate, sputum, etc.), 24.8% in blood, 18.7% in skin and soft tissues, 9.3% in nasal swabs, 5.4% in urine, 4.1% in ears, and 1% in sterile body fluid. Using PCR, mecC was not identified in any of the S. aureus strains isolated from different clinical microbiology laboratories. mecA gene positivity was found in 315 of the MRSA strains (63.8%). Staphylococcal Cassette Chromosome mec (SCCmec) type was identified in 232 strains (46.9%), of which 136 (58.7%) were type II, 75 (32.4%) were type IV, 12 (5.1%) were type IIIb, six (2.5%) were type I, and three (1.3%) were type III. Conclusion: This is the first multi-centered study to investigate MRSA strains isolated from different regions in Turkey. The mecC gene was not detected in any of the MRSA strains. We believe that this study will constitute an important basis for monitoring possible future changes. Keywords: Methicillin resistance, Staphylococcus aureus, mecC, mecA, Turkey
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1876-0341
Relation: http://www.sciencedirect.com/science/article/pii/S1876034119300656; https://doaj.org/toc/1876-0341
DOI: 10.1016/j.jiph.2019.01.063
URL الوصول: https://doaj.org/article/7bec4e3c3c744390ba6198b77d0a9143
رقم الأكسشن: edsdoj.7bec4e3c3c744390ba6198b77d0a9143
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:18760341
DOI:10.1016/j.jiph.2019.01.063