دورية أكاديمية
A Novel Hydroxyapatite/Vitamin B12 Nanoformula for Treatment of Bone Damage: Preparation, Characterization, and Anti-Arthritic, Anti-Inflammatory, and Antioxidant Activities in Chemically Induced Arthritic Rats
| العنوان: | A Novel Hydroxyapatite/Vitamin B12 Nanoformula for Treatment of Bone Damage: Preparation, Characterization, and Anti-Arthritic, Anti-Inflammatory, and Antioxidant Activities in Chemically Induced Arthritic Rats |
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| المؤلفون: | Amany Belal, Rehab Mahmoud, Eman E. Mohamed, Ahmed Farghali, Fatma I. Abo El-Ela, Amr Gamal, Fatma Mohamed Halfaya, Esraa Khaled, Abdelbasset A. Farahat, Ahmed H. E. Hassan, Mohammed M. Ghoneim, Mohamed Taha, Mohamed Y. Zaky |
| المصدر: | Pharmaceuticals, Vol 16, Iss 4, p 551 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | bone damage, bone disability research, rheumatoid arthritis, nano-HAP/Vit B12, anti-arthritic, antioxidant, Medicine, Pharmacy and materia medica, RS1-441 |
| الوصف: | The usage of nanomaterials for rheumatoid arthritis (RA) treatment can improve bioavailability and enable selective targeting. The current study prepares and evaluates the in vivo biological effects of a novel hydroxyapatite/vitamin B12 nanoformula in Complete Freund’s adjuvant-induced arthritis in rats. The synthesized nanoformula was characterized using XRD, FTIR, BET analysis, HERTEM, SEM, particle size, and zeta potential. We synthesized pure HAP NPs with 71.01% loading weight percentages of Vit B12 and 49 mg/g loading capacity. Loading of vitamin B12 on hydroxyapatite was modeled by Monte Carlo simulation. Anti-arthritic, anti-inflammatory, and antioxidant effects of the prepared nanoformula were assessed. Treated arthritic rats showed lower levels of RF and CRP, IL-1β, TNF-α, IL-17, and ADAMTS-5, but higher IL-4 and TIMP-3 levels. In addition, the prepared nanoformula increased GSH content and GST antioxidant activity while decreasing LPO levels. Furthermore, it reduced the expression of TGF-β mRNA. Histopathological examinations revealed an improvement in joint injuries through the reduction of inflammatory cell infiltration, cartilage deterioration, and bone damage caused by Complete Freund’s adjuvant. These findings indicate that the anti-arthritic, antioxidant, and anti-inflammatory properties of the prepared nanoformula could be useful for the development of new anti-arthritic treatments. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1424-8247 |
| Relation: | https://www.mdpi.com/1424-8247/16/4/551; https://doaj.org/toc/1424-8247 |
| DOI: | 10.3390/ph16040551 |
| URL الوصول: | https://doaj.org/article/d7bf8af8c4564168b3c719ba799a8ada |
| رقم الأكسشن: | edsdoj.7bf8af8c4564168b3c719ba799a8ada |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14248247 |
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| DOI: | 10.3390/ph16040551 |