دورية أكاديمية
Genetic screen identifies a requirement for SMN in mRNA localisation within the Drosophila oocyte
| العنوان: | Genetic screen identifies a requirement for SMN in mRNA localisation within the Drosophila oocyte |
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| المؤلفون: | Beppe Aquilina, Ruben J. Cauchi |
| المصدر: | BMC Research Notes, Vol 11, Iss 1, Pp 1-6 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| مصطلحات موضوعية: | Survival motor neuron, SMN, Genetic screen, Spinal muscular atrophy, mRNA localisation, Gurken, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| الوصف: | Abstract Objective Spinal muscular atrophy (SMA) results from insufficient levels of the survival motor neuron (SMN) protein. Drosophila is conducive to large-scale genetic-modifier screens which can reveal novel pathways underpinning the disease mechanism. We tested the ability of a large collection of genomic deletions to enhance SMN-dependent lethality. To test our design, we asked whether our study can identify loci containing genes identified in previous genetic screens. Our objective was to find a common link between genes flagged in independent screens, which would allow us to expose novel functions for SMN in vivo. Results Out of 128 chromosome deficiency lines, 12 (9.4%) were found to consistently depress adult viability when crossed to SMN loss-of-function heterozygotes. In their majority, the enhancing deletions harboured genes that were previously identified as genetic modifiers, hence, validating the design of the screen. Importantly, gene overlap allowed us to flag genes with a role in post-transcriptional regulation of mRNAs that are crucial for determining the axes of the oocyte and future embryo. We find that SMN is also required for the correct localisation of gurken and oskar mRNAs in oocytes. These findings extend the role of SMN in oogenesis by identifying a key requirement for mRNA trafficking. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-0500 |
| Relation: | http://link.springer.com/article/10.1186/s13104-018-3496-1; https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-018-3496-1 |
| URL الوصول: | https://doaj.org/article/7d92fdcef04a4be89833a2deec6ca5e0 |
| رقم الأكسشن: | edsdoj.7d92fdcef04a4be89833a2deec6ca5e0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17560500 |
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| DOI: | 10.1186/s13104-018-3496-1 |