دورية أكاديمية
Cell Versus Chemokine Therapy Effects on Cell Mobilization to Chronically Dysfunctional Urinary Sphincters of Nonhuman Primates
| العنوان: | Cell Versus Chemokine Therapy Effects on Cell Mobilization to Chronically Dysfunctional Urinary Sphincters of Nonhuman Primates |
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| المؤلفون: | J. Koudy Williams, Silmi Mariya, Irma Suparto, Shannon S. Lankford, Karl-Erik Andersson |
| المصدر: | International Neurourology Journal, Vol 22, Iss 4, Pp 260-267 (2018) |
| بيانات النشر: | Korean Continence Society, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Lentivirus transduction, Urinary sphincter, Cell mobilization, Stem cells, Chemokines, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Purpose A major question remaining in approaches to tissue engineering and organ replacement is the role of native mobilized native cells in the regeneration process of damaged tissues and organs. The goal of this study was to compare the cell mobilizing effects of the chemokine CXCL12 and cell therapy on the urinary sphincter of nonhuman primates (NHP) with chronic intrinsic urinary sphincter dysfunction. Methods Either autologous lenti-M-cherry labeled skeletal muscle precursor cells (skMPCs) or CXCL12 were injected directly into the sphincter complex of female NHPs with or without surgery-induced chronic urinary sphincter dysfunction (n=4/treatment condition). All monkeys had partial bone marrow transplantation with autologous lenti-green fluorescent protein (GFP) bone marrow cells prior to treatment. Labeled cells were identified, characterized and quantified using computer-assisted immunohistochemistry 6 months posttreatment. Results GFP-labeled bone marrow cells (BMCs) were identified in the bone marrow and both BMCs and skMPCs were found in the urinary sphincter at 6-month postinjection. BMCs and skMPCs were present in the striated muscle, smooth muscle, and lamina propria/urothelium of the sphincter tissue. Sphincter injury increased the sphincter content of BMCs when analyzed 6-month postinjection. CXCL12 treatment, but not skMPCs, increased the number of BMCs in all layers of the sphincter complex (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2093-4777 2093-6931 |
| Relation: | http://www.einj.org/upload/pdf/inj-1836126-063.pdf; https://doaj.org/toc/2093-4777; https://doaj.org/toc/2093-6931 |
| DOI: | 10.5213/inj.1836126.063 |
| URL الوصول: | https://doaj.org/article/7dacfd82a9fa4d3ea8e895153e9dcb55 |
| رقم الأكسشن: | edsdoj.7dacfd82a9fa4d3ea8e895153e9dcb55 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20934777 20936931 |
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| DOI: | 10.5213/inj.1836126.063 |