دورية أكاديمية
Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia
| العنوان: | Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia |
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| المؤلفون: | Jiawen Yong, Sabine Gröger, Julia von Bremen, Joerg Meyle, Sabine Ruf |
| المصدر: | Cells, Vol 11, Iss 15, p 2350 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | cementoblasts, hypoxia, PD-L1, HIF-1α, orthodontic, immunorthodontics, Cytology, QH573-671 |
| الوصف: | Recent studies have revealed that hypoxia alters the PD-L1 expression in periodontal cells. HIF-1α is a key regulator for PD-L1. As hypoxia presents a hallmark of an orthodontically induced microenvironment, hypoxic stimulation of PD-L1 expression may play vital roles in immunorthodontics and orthodontically induced inflammatory root resorption (OIIRR). This study aims to investigate the hypoxic regulation of PD-L1 in cementoblasts, and its interaction with hypoxia-induced HIF-1α expression. The cementoblast (OCCM-30) cells (M. Somerman, NIH, NIDCR, Bethesda, Maryland) were cultured in the presence and absence of cobalt (II) chloride (CoCl2). Protein expression of PD-L1 and HIF-1α as well as their gene expression were evaluated by Western blotting and RT-qPCR. Immunofluorescence was applied to visualize the localization of the proteins within cells. The HIF-1α inhibitor (HY-111387, MedChemExpress) was added, and CRISPR/Cas9 plasmid targeting HIF-1α was transferred for further investigation by flow cytometry analysis. Under hypoxic conditions, cementoblasts undergo an up-regulation of PD-L1 expression at protein and mRNA levels. Silencing of HIF-1α using CRISPR/Cas9 indicated a major positive correlation with HIF-1α in regulating PD-L1 expression. Taken together, these findings show the influence of hypoxia on PD-L1 expression is modulated in a HIF-1α dependent manner. The HIF-1α/PD-L1 pathway may play a role in the immune response of cementoblasts. Thus, combined HIF-1α/PD-L1 inhibition could be of possible therapeutic relevance for OIIRR prevention. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2073-4409 88769690 |
| Relation: | https://www.mdpi.com/2073-4409/11/15/2350; https://doaj.org/toc/2073-4409 |
| DOI: | 10.3390/cells11152350 |
| URL الوصول: | https://doaj.org/article/7ddb08ec18824be4ba87ab887696904e |
| رقم الأكسشن: | edsdoj.7ddb08ec18824be4ba87ab887696904e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20734409 88769690 |
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| DOI: | 10.3390/cells11152350 |