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Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

التفاصيل البيبلوغرافية
العنوان: Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
المؤلفون: Benjamin D Engel, Miroslava Schaffer, Luis Kuhn Cuellar, Elizabeth Villa, Jürgen M Plitzko, Wolfgang Baumeister
المصدر: eLife, Vol 4 (2015)
بيانات النشر: eLife Sciences Publications Ltd, 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Chlamydomonas, focused ion beam, cryo-electron tomography, chloroplast, thylakoid, RuBisCO, Medicine, Science, Biology (General), QH301-705.5
الوصف: Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional structures of the chloroplast in its native state within the cell. Chloroplast envelope inner membrane invaginations were frequently found in close association with thylakoid tips, and the tips of multiple thylakoid stacks converged at dynamic sites on the chloroplast envelope, implicating lipid transport in thylakoid biogenesis. Subtomogram averaging and nearest neighbor analysis revealed that RuBisCO complexes were hexagonally packed within the pyrenoid, with ∼15 nm between their centers. Thylakoid stacks and the pyrenoid were connected by cylindrical pyrenoid tubules, physically bridging the sites of light-dependent photosynthesis and light-independent carbon fixation. Multiple parallel minitubules were bundled within each pyrenoid tubule, possibly serving as conduits for the targeted one-dimensional diffusion of small molecules such as ATP and sugars between the chloroplast stroma and the pyrenoid matrix.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
81750374
Relation: https://elifesciences.org/articles/04889; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.04889
URL الوصول: https://doaj.org/article/aa7ddddf6379430a81750374cc73fc4c
رقم الأكسشن: edsdoj.7ddddf6379430a81750374cc73fc4c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
81750374
DOI:10.7554/eLife.04889