دورية أكاديمية
Engineered EVs for Oxidative Stress Protection
| العنوان: | Engineered EVs for Oxidative Stress Protection |
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| المؤلفون: | Anna Maria Tolomeo, Santina Quarta, Alessandra Biasiolo, Mariagrazia Ruvoletto, Michela Pozzobon, Giada De Lazzari, Ricardo Malvicini, Cristian Turato, Giorgio Arrigoni, Patrizia Pontisso, Maurizio Muraca |
| المصدر: | Pharmaceuticals, Vol 14, Iss 8, p 703 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | extracellular vesicles, SerpinB3, oxidative stress, cytoprotection, Medicine, Pharmacy and materia medica, RS1-441 |
| الوصف: | Extracellular vesicles (EVs) are increasingly studied as vectors for drug delivery because they can transfer a variety of molecules across biological barriers. SerpinB3 is a serine protease inhibitor that has shown a protective anti-apoptotic function in a variety of stressful conditions. The aim of this study was to evaluate protection from oxidative stress-induced damage, using extracellular vesicles that overexpress SerpinB3 (EVs-SB3) in order to enhance the effect of extracellular vesicles on cellular homeostasis. EVs-SB3s were obtained from HepG2 cells engineered to overexpress SerpinB3 and they revealed significant proteomic changes, mostly characterized by a reduced expression of other proteins compared with EVs from non-engineered cells. These EV preparations showed a significantly higher protection from H2O2 induced oxidative stress in both the hepatoma cell line and in primary cardiomyocytes, compared to cells treated with naïve EVs or SerpinB3 alone, used at the same concentration. In conclusion, the induction of SerpinB3 transgene expression results in the secretion of EVs enriched with the protein product that exhibits enhanced cytoprotective activity, compared with naïve EVs or the nude SerpinB3 protein. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1424-8247 |
| Relation: | https://www.mdpi.com/1424-8247/14/8/703; https://doaj.org/toc/1424-8247 |
| DOI: | 10.3390/ph14080703 |
| URL الوصول: | https://doaj.org/article/7e84797b475a443e8b4f24d7cedbd8e0 |
| رقم الأكسشن: | edsdoj.7e84797b475a443e8b4f24d7cedbd8e0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14248247 |
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| DOI: | 10.3390/ph14080703 |