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Thermo-responsive cell culture carriers based on poly(vinyl methyl ether)—the effect of biomolecular ligands to balance cell adhesion and stimulated detachment

التفاصيل البيبلوغرافية
العنوان: Thermo-responsive cell culture carriers based on poly(vinyl methyl ether)—the effect of biomolecular ligands to balance cell adhesion and stimulated detachment
المؤلفون: Juliane Teichmann, Mirko Nitschke, Dagmar Pette, Monika Valtink, Stefan Gramm, Frauke V Härtel, Thomas Noll, Richard H W Funk, Katrin Engelmann, Carsten Werner
المصدر: Science and Technology of Advanced Materials, Vol 16, Iss 4 (2015)
بيانات النشر: Taylor & Francis Group, 2015.
سنة النشر: 2015
المجموعة: LCC:Materials of engineering and construction. Mechanics of materials
LCC:Biotechnology
مصطلحات موضوعية: thermo-responsive polymer, physico-chemical properties, biomolecular functionalization, poly(n-isopropylacrylamide), corneal endothelial cell sheet, tissue engineering, endothelial transplantation, Materials of engineering and construction. Mechanics of materials, TA401-492, Biotechnology, TP248.13-248.65
الوصف: Two established material systems for thermally stimulated detachment of adherent cells were combined in a cross-linked polymer blend to merge favorable properties. Through this approach poly(N-isopropylacrylamide) (PNiPAAm) with its superior switching characteristic was paired with a poly(vinyl methyl ether)-based composition that allows adjusting physico-chemical and biomolecular properties in a wide range. Beyond pure PNiPAAm, the proposed thermo-responsive coating provides thickness, stiffness and swelling behavior, as well as an apposite density of reactive sites for biomolecular functionalization, as effective tuning parameters to meet specific requirements of a particular cell type regarding initial adhesion and ease of detachment. To illustrate the strength of this approach, the novel cell culture carrier was applied to generate transplantable sheets of human corneal endothelial cells (HCEC). Sheets were grown, detached, and transferred onto planar targets. Cell morphology, viability and functionality were analyzed by immunocytochemistry and determination of transepithelial electrical resistance (TEER) before and after sheet detachment and transfer. HCEC layers showed regular morphology with appropriate TEER. Cells were positive for function-associated marker proteins ZO-1, Na+/K+-ATPase, and paxillin, and extracellular matrix proteins fibronectin, laminin and collagen type IV before and after transfer. Sheet detachment and transfer did not impair cell viability. Subsequently, a potential application in ophthalmology was demonstrated by transplantation onto de-endothelialized porcine corneas in vitro. The novel thermo-responsive cell culture carrier facilitates the generation and transfer of functional HCEC sheets. This paves the way to generate tissue engineered human corneal endothelium as an alternative transplant source for endothelial keratoplasty.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1468-6996
1878-5514
Relation: https://doaj.org/toc/1468-6996; https://doaj.org/toc/1878-5514
DOI: 10.1088/1468-6996/16/4/045003
URL الوصول: https://doaj.org/article/7e8606b500264993bc21f53f20df568d
رقم الأكسشن: edsdoj.7e8606b500264993bc21f53f20df568d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14686996
18785514
DOI:10.1088/1468-6996/16/4/045003