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Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing

التفاصيل البيبلوغرافية
العنوان: Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing
المؤلفون: Yongqiang Miao, Xueliang Zhao, Jianlin Lei, Jingru Ding, Hang Feng, Ke Wu, Jiaohu Liu, Chunyang Wang, Dongyang Ye, Xinglong Wang, Juan Wang, Zengqi Yang
المصدر: Animals, Vol 13, Iss 17, p 2763 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Veterinary medicine
LCC:Zoology
مصطلحات موضوعية: Hu sheep, pneumonia, Mannheimia, microbiome, 16SrRNA sequencing, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: Hu sheep, a locally bred species in China known for its high productivity, is currently suffering from pneumonia. Here, we combine high-throughput 16SrRNA gene sequencing and bacterial culturing to examine the bacterial community in pneumonic Hu Sheep lungs (p < 0.05). The results showed that the abundance and diversity of lung bacteria in healthy sheep were significantly higher than those in pneumonia sheep (p = 0.139), while there was no significant difference between moderate and severe pneumonia. Furthermore, the composition of the lung microbiota community underwent significant alterations between different levels of pneumonia severity. The application of LEfSe analysis revealed a notable enrichment of Mannheimiae within the lungs of sheep afflicted with moderate pneumonia (p < 0.01), surpassing the levels observed in their healthy counterparts. Additionally, Fusobacterium emerged as the prevailing bacterial group within the lungs of sheep suffering from severe pneumonia. Integrating the results of bacterial isolation and identification, we conclusively determined that Mannheimia haemolytica was the primary pathogenic bacterium within the lungs of sheep afflicted with moderate pneumonia. Furthermore, the exacerbation of pneumonia may be attributed to the synergistic interplay between Fusobacterium spp. and other bacterial species. Our results provide new insights for guiding preventive and therapeutic measures for pneumonia of different severities in sheep.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-2615
Relation: https://www.mdpi.com/2076-2615/13/17/2763; https://doaj.org/toc/2076-2615
DOI: 10.3390/ani13172763
URL الوصول: https://doaj.org/article/7f7a2c42a8de42ebb6ed6ba69340207c
رقم الأكسشن: edsdoj.7f7a2c42a8de42ebb6ed6ba69340207c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20762615
DOI:10.3390/ani13172763