دورية أكاديمية
أعرض في EDS

p63 attenuates epithelial to mesenchymal potential in an experimental prostate cell model.

التفاصيل البيبلوغرافية
العنوان: p63 attenuates epithelial to mesenchymal potential in an experimental prostate cell model.
المؤلفون: Jan Roger Olsen, Anne Margrete Oyan, Kari Rostad, Margrete R Hellem, Jie Liu, Lisha Li, David R Micklem, Hallvard Haugen, James B Lorens, Varda Rotter, Xi-Song Ke, Biaoyang Lin, Karl-Henning Kalland
المصدر: PLoS ONE, Vol 8, Iss 5, p e62547 (2013)
بيانات النشر: Public Library of Science (PLoS), 2013.
سنة النشر: 2013
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The transcription factor p63 is central for epithelial homeostasis and development. In our model of epithelial to mesenchymal transition (EMT) in human prostate cells, p63 was one of the most down-regulated transcription factors during EMT. We therefore investigated the role of p63 in EMT. Over-expression of the predominant epithelial isoform ΔNp63α in mesenchymal type cells of the model led to gain of several epithelial characteristics without resulting in a complete mesenchymal to epithelial transition (MET). This was corroborated by a reciprocal effect when p63 was knocked down in epithelial EP156T cells. Global gene expression analyses showed that ΔNp63α induced gene modules involved in both cell-to-cell and cell-to-extracellular-matrix junctions in mesenchymal type cells. Genome-wide analysis of p63 binding sites using ChIP-seq analyses confirmed binding of p63 to regulatory areas of genes associated with cell adhesion in prostate epithelial cells. DH1 and ZEB1 are two elemental factors in the control of EMT. Over-expression and knock-down of these factors, respectively, were not sufficient alone or in combination with ΔNp63α to reverse completely the mesenchymal phenotype. The partial reversion of epithelial to mesenchymal transition might reflect the ability of ΔNp63α, as a key co-ordinator of several epithelial gene expression modules, to reduce epithelial to mesenchymal plasticity (EMP). The utility of ΔNp63α expression and the potential of reduced EMP in order to counteract metastasis warrant further investigation.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3641034?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0062547
URL الوصول: https://doaj.org/article/d7fd1bb4aff245c3a9fcd44b2011fe53
رقم الأكسشن: edsdoj.7fd1bb4aff245c3a9fcd44b2011fe53
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0062547