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Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2.

التفاصيل البيبلوغرافية
العنوان: Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2.
المؤلفون: Yuichi Morozumi, Ryohei Ino, Shukuko Ikawa, Naozumi Mimida, Takeshi Shimizu, Seiichi Toki, Hiroaki Ichikawa, Takehiko Shibata, Hitoshi Kurumizaka
المصدر: PLoS ONE, Vol 8, Iss 10, p e75451 (2013)
بيانات النشر: Public Library of Science (PLoS), 2013.
سنة النشر: 2013
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: In higher eukaryotes, RAD51 functions as an essential protein in homologous recombination and recombinational repair of DNA double strand breaks. During these processes, RAD51 catalyzes homologous pairing between single-stranded DNA and double-stranded DNA. Japonica cultivars of rice (Oryza sativa) encode two RAD51 proteins, RAD51A1 and RAD51A2, whereas only one RAD51 exists in yeast and mammals. However, the functional differences between RAD51A1 and RAD51A2 have not been elucidated, because their biochemical properties have not been characterized. In the present study, we purified RAD51A1 and RAD51A2, and found that RAD51A2 robustly promotes homologous pairing in vitro. RAD51A1 also possesses homologous-pairing activity, but it is only about 10% of the RAD51A2 activity. Both RAD51A1 and RAD51A2 bind to ssDNA and dsDNA, and their DNA binding strictly requires ATP, which modulates the polymer formation activities of RAD51A1 and RAD51A2. These findings suggest that although both RAD51A1 and RAD51A2 have the potential to catalyze homologous pairing, RAD51A2 may be the major recombinase in rice.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3790826?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0075451
URL الوصول: https://doaj.org/article/8010c413bfae4398a9cf46861e26e8e4
رقم الأكسشن: edsdoj.8010c413bfae4398a9cf46861e26e8e4
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0075451