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3D printing of interferon γ-preconditioned NSC-derived exosomes/collagen/chitosan biological scaffolds for neurological recovery after TBI

التفاصيل البيبلوغرافية
العنوان: 3D printing of interferon γ-preconditioned NSC-derived exosomes/collagen/chitosan biological scaffolds for neurological recovery after TBI
المؤلفون: Chong Chen, Zhe-Han Chang, Bin Yao, Xiao-Yin Liu, Xiao-Wang Zhang, Jun Liang, Jing-Jing Wang, Shuang-Qing Bao, Meng-Meng Chen, Ping Zhu, Xiao-Hong Li
المصدر: Bioactive Materials, Vol 39, Iss , Pp 375-391 (2024)
بيانات النشر: KeAi Communications Co., Ltd., 2024.
سنة النشر: 2024
المجموعة: LCC:Materials of engineering and construction. Mechanics of materials
LCC:Biology (General)
مصطلحات موضوعية: Exosomes, Traumatic brain injury, Interferon γ, 3D printing, Neural reconstruction, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5
الوصف: The reconstruction of neural function and recovery of chronic damage following traumatic brain injury (TBI) remain significant clinical challenges. Exosomes derived from neural stem cells (NSCs) offer various benefits in TBI treatment. Numerous studies confirmed that appropriate preconditioning methods enhanced the targeted efficacy of exosome therapy. Interferon-gamma (IFN-γ) possesses immunomodulatory capabilities and is widely involved in neurological disorders. In this study, IFN-γ was employed for preconditioning NSCs to enhance the efficacy of exosome (IFN-Exo, IE) for TBI. miRNA sequencing revealed the potential of IFN-Exo in promoting neural differentiation and modulating inflammatory responses. Through low-temperature 3D printing, IFN-Exo was combined with collagen/chitosan (3D-CC-IE) to preserve the biological activity of the exosome. The delivery of exosomes via biomaterial scaffolds benefited the retention and therapeutic potential of exosomes, ensuring that they could exert long-term effects at the injury site. The 3D-CC-IE scaffold exhibited excellent biocompatibility and mechanical properties. Subsequently, 3D-CC-IE scaffold significantly improved impaired motor and cognitive functions after TBI in rat. Histological results showed that 3D-CC-IE scaffold markedly facilitated the reconstruction of damaged neural tissue and promoted endogenous neurogenesis. Further mechanistic validation suggested that IFN-Exo alleviated neuroinflammation by modulating the MAPK/mTOR signaling pathway. In summary, the results of this study indicated that 3D-CC-IE scaffold engaged in long-term pathophysiological processes, fostering neural function recovery after TBI, offering a promising regenerative therapy avenue.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2452-199X
Relation: http://www.sciencedirect.com/science/article/pii/S2452199X24001920; https://doaj.org/toc/2452-199X
DOI: 10.1016/j.bioactmat.2024.05.026
URL الوصول: https://doaj.org/article/801f2ccd0e82468b8d0b540be908319e
رقم الأكسشن: edsdoj.801f2ccd0e82468b8d0b540be908319e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2452199X
DOI:10.1016/j.bioactmat.2024.05.026