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Effects of total coumarins from Pileostegia tomentella on exosomal miRNA expression and angiogenesis in colorectal cancer cells

التفاصيل البيبلوغرافية
العنوان: Effects of total coumarins from Pileostegia tomentella on exosomal miRNA expression and angiogenesis in colorectal cancer cells
المؤلفون: Ying Liu, Dao-hai Cheng, Zheng-ying Su, Ji-hua Lv, Li Wang, Yu-yin Deng, Li Li
المصدر: Pharmaceutical Biology, Vol 62, Iss 1, Pp 153-161 (2024)
بيانات النشر: Taylor & Francis Group, 2024.
سنة النشر: 2024
المجموعة: LCC:Therapeutics. Pharmacology
مصطلحات موضوعية: Exosome, miR-375-3p, tumour metastasis, Therapeutics. Pharmacology, RM1-950
الوصف: AbstractContext Pileostegia tomentella Hand. Mazz (Saxifragaceae) total coumarins (TCPT) show antitumour activity in colorectal cancer (CRC) with unknown mechanism of action. Tumour angiogenesis mediated by exosomes-derived miRNA exhibits the vital regulation of endothelial cell function in metastasis of CRC.Objective To investigate the effect of TCPT on exosomal miRNA expression and angiogenesis of CRC cells.Materials and methods HT-29-derived exosomes were generated from human CRC cells (HT-29) or either treated with TCPT (100 μg/mL) for 24 h, followed by identification by transmission electron microscope, nanoparticle tracking analysis (NTA) and Western blot. Co-culture experiments for human umbilical vein endothelial cells (HUVECs) and exosomes were performed to detect the uptake of exosomes in HUVECs and its influence on HUVECs cells migration and lumen formation ability. Potential target miRNAs in exosomes were screened out by sequencing technology. Rescue assays of angiogenesis were performed by the transfecting mimics or inhibitors of targeted miRNA into HUVECs.Results HT-29-derived exosomes, after TCPT treatment (Exo-TCPT), inhibited the migration and lumen formation of HUVECs, reduced the expression levels of vascular marker (FLT-1, VCAM-1 and VEGFR-2) in HUVECs. Furthermore, the level of miR-375-3p was significantly upregulated in Exo-TCPT. Rescue assays showed that high expression of miR-375-3p in HUVECs inhibited migration and lumen formation abilities, which was consistent with the effects of Exo-TCPT, whereas applying miR-375-3p inhibitors displayed opposite effects.Discussion and conclusion TCPT exhibits anti-angiogenesis in CRC, possibly through upregulating exosomal miR-375-3p. Our findings will shed light on new target exosomes miRNA-mediated tumour microenvironment and the therapeutic application of Pileostegia tomentella in CRC.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 13880209
1744-5116
1388-0209
Relation: https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116
DOI: 10.1080/13880209.2024.2309871
URL الوصول: https://doaj.org/article/805c6b46529f41a3b19a0373b43c7c09
رقم الأكسشن: edsdoj.805c6b46529f41a3b19a0373b43c7c09
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13880209
17445116
DOI:10.1080/13880209.2024.2309871