دورية أكاديمية
Ultrasound-triggered three dimensional hyaluronic acid hydrogel promotes in vitro and in vivo reprogramming into induced pluripotent stem cells
| العنوان: | Ultrasound-triggered three dimensional hyaluronic acid hydrogel promotes in vitro and in vivo reprogramming into induced pluripotent stem cells |
|---|---|
| المؤلفون: | Deogil Kim, Min-Ju Lee, Yoshie Arai, Jinsung Ahn, Gun Woo Lee, Soo-Hong Lee |
| المصدر: | Bioactive Materials, Vol 38, Iss , Pp 331-345 (2024) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| مصطلحات موضوعية: | Induced pluripotent stem cell, Cellular reprogramming, Low-intensity ultrasound, Biophysical stimulation, Cytoskeletal rearrangement, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| الوصف: | Cellular reprogramming technologies have been developed with different physicochemical factors to improve the reprogramming efficiencies of induced pluripotent stem cells (iPSCs). Ultrasound is a clinically applied noncontact biophysical factor known for regulating various cellular behaviors but remains uninvestigated for cellular reprogramming. Here, we present a new reprogramming strategy using low-intensity ultrasound (LIUS) to improve cellular reprogramming of iPSCs in vitro and in vivo. Under 3D microenvironment conditions, increased LIUS stimulation shows enhanced cellular reprogramming of the iPSCs. The cellular reprogramming process facilitated by LIUS is accompanied by increased mesenchymal to epithelial transition and histone modification. LIUS stimulation transiently modulates the cytoskeletal rearrangement, along with increased membrane fluidity and mobility to increase HA/CD44 interactions. Furthermore, LIUS stimulation with HA hydrogel can be utilized in application of both human cells and in vivo environment, for enhanced reprogrammed cells into iPSCs. Thus, LIUS stimulation with a combinatorial 3D microenvironment system can improve cellular reprogramming in vitro and in vivo environments, which can be applied in various biomedical fields. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2452-199X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2452199X24001774; https://doaj.org/toc/2452-199X |
| DOI: | 10.1016/j.bioactmat.2024.05.011 |
| URL الوصول: | https://doaj.org/article/80b032f3590d43b59ed50a89fc5fb0af |
| رقم الأكسشن: | edsdoj.80b032f3590d43b59ed50a89fc5fb0af |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2452199X |
|---|---|
| DOI: | 10.1016/j.bioactmat.2024.05.011 |