دورية أكاديمية
Rapid phosphorylation of glucose-6-phosphate dehydrogenase by casein kinase 2 sustains redox homeostasis under ionizing radiation
| العنوان: | Rapid phosphorylation of glucose-6-phosphate dehydrogenase by casein kinase 2 sustains redox homeostasis under ionizing radiation |
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| المؤلفون: | Yilong Hao, Tao Ren, Xiaoke Huang, Mi Li, Jong-Ho Lee, Qianming Chen, Rui Liu, Qingfeng Tang |
| المصدر: | Redox Biology, Vol 65, Iss , Pp 102810- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | G6PD, CK2, NADPH, Oxidative stress, Ionizing radiation, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Exposure to ionizing radiation leads to oxidative damages in living cells. NADPH provides the indispensable reducing power to regenerate the reduced glutathione to maintain cellular redox equilibria. In mammalian cells, pentose phosphate pathway (PPP) is the major route to produce NADPH by using glycolytic intermediates, and the rate-limiting step of PPP is controlled by glucose-6-phosphate dehydrogenase (G6PD). Nevertheless, whether G6PD is timely co-opted under ionizing radiation to cope with oxidative stress remains elusive. Here we show that cellular G6PD activity is induced 30 min after ionizing radiation, while its protein expression is mostly unchanged. Mechanistically, casein kinase 2 (CK2) phosphorylates G6PD T145 under ionizing radiation, which consolidates the enzymatic activity of G6PD by facilitating G6PD binding with its substrate NADP+. Further, CK2-dependent G6PD T145 phosphorylation promotes NADPH production, decreases ROS level and supports cell proliferation under ionizing radiation. Our findings report a new anti-oxidative signaling route under ionizing radiation, by which CK2-mediated rapid activation of G6PD orchestrates NADPH synthesis to maintain redox homeostasis, thereby highlighting its potential value in the early treatment of ionizing radiation-induced injuries. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-2317 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213231723002112; https://doaj.org/toc/2213-2317 |
| DOI: | 10.1016/j.redox.2023.102810 |
| URL الوصول: | https://doaj.org/article/8118c6a98a93409e8ce7213778a2f59f |
| رقم الأكسشن: | edsdoj.8118c6a98a93409e8ce7213778a2f59f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22132317 |
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| DOI: | 10.1016/j.redox.2023.102810 |