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Species specific differences in use of ANP32 proteins by influenza A virus

التفاصيل البيبلوغرافية
العنوان: Species specific differences in use of ANP32 proteins by influenza A virus
المؤلفون: Jason S Long, Alewo Idoko-Akoh, Bhakti Mistry, Daniel Goldhill, Ecco Staller, Jocelyn Schreyer, Craig Ross, Steve Goodbourn, Holly Shelton, Michael A Skinner, Helen Sang, Michael J McGrew, Wendy Barclay
المصدر: eLife, Vol 8 (2019)
بيانات النشر: eLife Sciences Publications Ltd, 2019.
سنة النشر: 2019
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Influenza, ANP32A, ANP32B, polymerase, gene editing, Medicine, Science, Biology (General), QH301-705.5
الوصف: Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/45066; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.45066
URL الوصول: https://doaj.org/article/8266bab1b21a4024ba777588e414ad75
رقم الأكسشن: edsdoj.8266bab1b21a4024ba777588e414ad75
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.45066