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Roots and stems of Kadsura coccinea extract induced developmental toxicity in zebrafish embryos/larvae through apoptosis and oxidative stress

التفاصيل البيبلوغرافية
العنوان: Roots and stems of Kadsura coccinea extract induced developmental toxicity in zebrafish embryos/larvae through apoptosis and oxidative stress
المؤلفون: Zhongshang Xia, Erwei Hao, Zhangmei Chen, Mingzhe Zhang, Yanting Wei, Man Wei, Xiaotao Hou, Zhengcai Du, Jiagang Deng
المصدر: Pharmaceutical Biology, Vol 58, Iss 1, Pp 1303-1310 (2020)
بيانات النشر: Taylor & Francis Group, 2020.
سنة النشر: 2020
المجموعة: LCC:Therapeutics. Pharmacology
مصطلحات موضوعية: hepatotoxicity, reactive oxygen species, malondialdehyde, herbal medicine, Therapeutics. Pharmacology, RM1-950
الوصف: Context Although the roots and stems of Kadsura coccinea (Lem.) A. C. Smith. [Schisandraceae] are herbs and traditional foods in Li nationality, its toxicity remains unclear. Objective To study developmental toxicity of K. coccinea consumption and explain underlying mechanisms. Materials and methods Zebrafish were applied to assess LC50 values of hydroethanol extract (KCH) and water extract (KCW) of Kadsura coccinea. In further study, three concentrations groups of KCH (3.75, 7.5 and 15 μg/mL for embryo, 7.5, 15 and 30 μg/mL for larvae) and control group (n = 30) were administered. At specific stages of zebrafish development, spontaneous movement, hatching rate, etc., were measured. Gene expressions related to developmental toxicity were examined. Results The LC50 value of KCH (24 or 45 μg/mL) was lower than KCW (1447 or 2011 μg/mL) in embryos or larvae. The inhibited spontaneous movement (20%), hatching rate (20%), body length (12%) and eye area (30%) were observed after KCH treatment. Moreover, the decreased liver areas (25%) and fluorescence intensity (33%), increased ALT (37%) and AST levels (42%) were found in larvae treated with KCH (30 μg/mL). The increased ROS (89%), MDA concentrations (30%), apoptosis generation (62%) and decreased T-SOD activity (16%) were also observed. The represented genes of developmental hepatotoxicity, oxidative stress and apoptosis in zebrafish were activated after KCH (15 or 30 μg/mL) treatment. Discussion and conclusions These results demonstrate that KCH has developmental toxicity on zebrafish. Our study provides a scientific basis for further research on the toxicity of Kadsura coccinea.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1388-0209
1744-5116
13880209
Relation: https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116
DOI: 10.1080/13880209.2020.1859553
URL الوصول: https://doaj.org/article/e82aca815a124904873c4762d92e8e17
رقم الأكسشن: edsdoj.82aca815a124904873c4762d92e8e17
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13880209
17445116
DOI:10.1080/13880209.2020.1859553