| الوصف: |
Gang Feng,1,2 Hui-Min Ma,1 Hou-Bao Huang,3 Ya-Wei Li,3 Peng Zhang,1 Jian-Jun Huang,1 Long Cheng,1,2 Guo-Rong Li4 1Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China; 2Anhui Province Key Laboratory of Active Biological Macro-molecules Research, Wannan Medical College, Wuhu 241002, Anhui, China; 3Department of Urology, The First Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China; 4Department of Urology, North Hospital, CHU of Saint-Etienne, Saint-Etienne 42055, France Background and aims: COL5A1 has been identified to be involved in metastasis of clear cell renal cell carcinoma (ccRCC) by bioinformatic analysis. This study aimed to investigate COL5A1 expression and its clinical significance in ccRCC. The function of COL5A1 in ccRCC was further investigated. Methods: COL5A1 expression was examined in 256 ccRCC tissues and paired adjacent normal renal tissues by immunohistochemistry and real-time quantitative PCR. The clinical significance of COL5A1 expression was evaluated. Downregulation of COL5A1 was achieved using siRNA. The effects of COL5A1 silencing on cell proliferation, apoptosis, migration, invasion in vitro, and tumor growth in vivo were investigated. Results: COL5A1 expression was upregulated in the majority of the ccRCC tissues at both protein and mRNA levels. COL5A1 expression was significantly correlated with tumor diameter, tumor stage, tumor grade, distant metastasis, recurrence, necrosis, and sarcomatoid (all P |
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