دورية أكاديمية
Molecular cloning and expression analysis of the mstn and myod genes in the hybrids derived from Megalobrama amblycephala × Xenocypris davidi Bleeker
| العنوان: | Molecular cloning and expression analysis of the mstn and myod genes in the hybrids derived from Megalobrama amblycephala × Xenocypris davidi Bleeker |
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| المؤلفون: | Siyu Fan, Ting Li, Zhong Tang, Zhifeng Zhou, Xin Deng, Lu Huang, Xinge Ouyang, Faxian Yu, Xiangqiong Yang, Liran Zhang, Min Tao |
| المصدر: | Reproduction and Breeding, Vol 4, Iss 3, Pp 134-144 (2024) |
| بيانات النشر: | KeAi Communications Co. Ltd., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Genetics LCC:Reproduction LCC:Animal biochemistry |
| مصطلحات موضوعية: | Distant hybridization, mstn, myod, Muscle growth and development, Genetics, QH426-470, Reproduction, QH471-489, Animal biochemistry, QP501-801 |
| الوصف: | The hybrids (BY) derived from blunt snout bream (Megalobrama amblycephala, BSB, ♀) and Bleeker's yellow tai (Xenocypris davidi Bleeker, YT, ♂) have a fast growth rate and body thickness. However, the molecular mechanisms underlying the growth advantage of BY are still unclear. MSTN, a member of the TGF-β superfamily, is a negative regulator of muscle growth and development. MYOD, a member of the myogenic regulatory factor family (MRFs), is a positive regulator of muscle growth and development. MSTN and MYOD interact to co-regulate muscle growth and development. In this study, we cloned the full-length cDNAs of mstn and myod in BY, analyzed differential expression of the two genes in muscle of BY and its parents, and observed the localization of the two genes during embryonic development of BY. Multiple sequence alignment of amino acids and phylogenetic analysis showed that mstn and myod were highly conserved in BY, BSB, YT, and other carp family fishes. Quantitative Real-time PCR (qRT-PCR) showed that mstn and myod were primarily expressed in muscle of BY, and there were significant differences in the expression of the two genes in the red and white muscles of BY, BSB, and YT at different age. The results of in situ hybridization of embryos showed an overlap in the temporal and spatial expression of mstn and myod in BY embryo development, suggesting that the two genes might interact to co-regulate embryo growth and development. This study provides a theoretical basis for further research on the mechanisms of mstn and myod in muscle growth of hybrid fishes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2667-0712 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2667071224000243; https://doaj.org/toc/2667-0712 |
| DOI: | 10.1016/j.repbre.2024.05.001 |
| URL الوصول: | https://doaj.org/article/83c1e50a6a2944deb68efc81896c3bfd |
| رقم الأكسشن: | edsdoj.83c1e50a6a2944deb68efc81896c3bfd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26670712 |
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| DOI: | 10.1016/j.repbre.2024.05.001 |