دورية أكاديمية
A paclitaxel prodrug nanoparticles with glutathion/reactive oxygen species dual‐responsive and CD206 targeting to improve the anti‐tumour effect
| العنوان: | A paclitaxel prodrug nanoparticles with glutathion/reactive oxygen species dual‐responsive and CD206 targeting to improve the anti‐tumour effect |
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| المؤلفون: | Changhai Wang, Yuwen Jiao, Xinyu Zhang, Mingxue Guo, Qing Zhang, Wenjun Hu, Shuang Dong, Tangthianchaichana Jakkree, Yang Lu, Jinling Wang |
| المصدر: | IET Nanobiotechnology, Vol 17, Iss 5, Pp 406-419 (2023) |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | cellular effects of radiation, drugs, drug delivery systems, nanoparticle, tumours, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract As a first‐line anticancer drug, paclitaxel has shortcomings, such as poor solubility and lack of tumour cell selectivity, which limit its further applications in clinical practice. Therefore, the authors aimed to utilise the characteristics of prodrug and nanotechnology to prepare a reactive oxygen species (ROS) and GSH dual‐responsive targeted tumour prodrug nanoparticle Man‐PEG‐SS‐PLGA/ProPTX to improve the clinical application status of paclitaxel limitation. The characterisation of Man‐PEG‐SS‐PLGA/ProPTX was carried out through preparation. The cytotoxicity of nanoparticles on tumour cells and the effect on apoptosis of tumour cells were investigated by cytotoxicity assay and flow cytometry analysis. The ROS responsiveness of nanoparticles was investigated by detecting the ROS level of tumour cells. The tumour cell selectivity of the nanoparticles was further investigated by receptor affinity assay and cell uptake assay. The particle size of Man‐PEG‐SS‐PLGA/ProPTX was (132.90 ± 1.81) nm, the dispersion coefficient Polymer dispersity index was 0.13 ± 0.03, and the Zeta potential was (−8.65 ± 0.50) mV. The encapsulation rate was 95.46 ± 2.31% and the drug load was 13.65 ± 2.31%. The nanoparticles could significantly inhibit the proliferation and promote apoptosis of MCF‐7, HepG2, and MDA‐MB‐231 tumour cells. It has good ROS response characteristics and targeting. The targeted uptake mechanism is energy‐dependent and endocytosis is mediated by non‐clathrin, non‐caveolin, lipid raft/caveolin, and cyclooxygenase (COX)/caveolin with a certain concentration dependence and time dependence. Man‐PEG‐SS‐PLGA/ProPTX is a tumour microenvironment‐responsive nanoparticle that can actively target tumour cells. It restricts the release of PTX in normal tissues, enhances its selectivity to tumour cells, and has significant antitumour activity, which is expected to solve the current limitations of PTX use. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1751-875X 1751-8741 |
| Relation: | https://doaj.org/toc/1751-8741; https://doaj.org/toc/1751-875X |
| DOI: | 10.1049/nbt2.12119 |
| URL الوصول: | https://doaj.org/article/a83e4512c43a4b89aac0a25b90d4a444 |
| رقم الأكسشن: | edsdoj.83e4512c43a4b89aac0a25b90d4a444 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1751875X 17518741 |
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| DOI: | 10.1049/nbt2.12119 |