دورية أكاديمية
Anti-oxidative effects of broccoli (Brassica oleracea var. italica) sprout extract in RAW 264.7 cell and cisplatin-induced testicular damage
| العنوان: | Anti-oxidative effects of broccoli (Brassica oleracea var. italica) sprout extract in RAW 264.7 cell and cisplatin-induced testicular damage |
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| المؤلفون: | Won-Young Lee, Hyun-Woo Shim, Hyun-Jung Park |
| المصدر: | Journal of Animal Reproduction and Biotechnology, Vol 38, Iss 4, Pp 189-198 (2023) |
| بيانات النشر: | The Korean Society of Animal Reproduction and Biotechnology, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medicine (General) LCC:Internal medicine |
| مصطلحات موضوعية: | antioxidation, broccoli sprouts, cisplatin, reactive oxygen stress, Biotechnology, TP248.13-248.65, Medicine (General), R5-920, Internal medicine, RC31-1245 |
| الوصف: | Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2, Gsr, HO-1, and catalase, were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2, HO-1, and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Korean |
| تدمد: | 2671-4639 2671-4663 |
| Relation: | https://www.e-jarb.org/journal/view.html?uid=2670&vmd=Full; https://doaj.org/toc/2671-4639; https://doaj.org/toc/2671-4663 |
| DOI: | 10.12750/JARB.38.4.189 |
| URL الوصول: | https://doaj.org/article/83f0dfb3288d4e419d33239b34108bcd |
| رقم الأكسشن: | edsdoj.83f0dfb3288d4e419d33239b34108bcd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26714639 26714663 |
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| DOI: | 10.12750/JARB.38.4.189 |