دورية أكاديمية
RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation
| العنوان: | RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation |
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| المؤلفون: | Xiaoyu Ning, Cong Huang, Changhong Dong, Jisu Jin, Xi Qiao, Jianying Guo, Wanqiang Qian, Fengqin Cao, Fanghao Wan |
| المصدر: | Frontiers in Ecology and Evolution, Vol 11 (2023) |
| بيانات النشر: | Frontiers Media S.A., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Evolution LCC:Ecology |
| مصطلحات موضوعية: | Agasicles hygrophila, odorant receptor co-receptor, RNA interference, mate recognition, host finding, Evolution, QH359-425, Ecology, QH540-549.5 |
| الوصف: | Alligator weed Alternanthera philoxeroides is a perennial, worldwide pernicious weed. The beetle Agasicles hygrophila is considered to be a classical biological agent used to control A. philoxeroides. In the insect peripheral olfactory system, the odorant receptor co-receptor (ORco) plays an important function in the perception of odors in insects. However, the function of ORco in the mating and host-finding behaviors of A. hygrophila remains unclear. In this study, we characterized the odorant receptor co-receptor of A. hygrophila (AhygOrco). Real-time quantitative PCR (qRT–PCR) showed that AhygOrco was predominantly expressed in the antennae of both male and female adults, and the difference between male and female antennae was not significant. The RNA interference (RNAi) results showed that compared to the control, the injection of AhygOrco dsRNA strongly reduced the expression of AhygOrco by 90% in male beetles and 89% in female beetles. The mate-seeking and feeding behavior of AhygOrco-silenced beetles were significantly inhibited. Male adults were significantly less successful in finding a mate compared to the control group. Furthermore, host allocation abilities toward A. philoxeroides of both adults were significantly repressed. These results indicated that AhygOrco is associated with A. hygrophila feeding and mate-seeking and that inhibition of AhygOrco expression is one of the causes of reduced host and mate recognition in A. hygrophila. Meanwhile, the study provides support for exploring gene functions based on RNAi. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2296-701X |
| Relation: | https://www.frontiersin.org/articles/10.3389/fevo.2023.1104962/full; https://doaj.org/toc/2296-701X |
| DOI: | 10.3389/fevo.2023.1104962 |
| URL الوصول: | https://doaj.org/article/e843e60ca4e242028a7f5221e7d6e00b |
| رقم الأكسشن: | edsdoj.843e60ca4e242028a7f5221e7d6e00b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2296701X |
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| DOI: | 10.3389/fevo.2023.1104962 |