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Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

التفاصيل البيبلوغرافية
العنوان: Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes
المؤلفون: Olena S. Oliinyk, Konstantin G. Chernov, Vladislav V. Verkhusha
المصدر: International Journal of Molecular Sciences, Vol 18, Iss 8, p 1691 (2017)
بيانات النشر: MDPI AG, 2017.
سنة النشر: 2017
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: bacterial photoreceptor, near-infrared fluorescent protein, phytochrome, cyanobacteriochrome, allophycocyanin, tetrapyrrole, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/18/8/1691; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms18081691
URL الوصول: https://doaj.org/article/8449802ff0734c2b83ff9fdd29ff9b46
رقم الأكسشن: edsdoj.8449802ff0734c2b83ff9fdd29ff9b46
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms18081691