دورية أكاديمية
Optimising effective parameters to improve performance quality in lateral flow immunoassay for detection of PBP2a in methicillin-resistant Staphylococcus aureus (MRSA)
| العنوان: | Optimising effective parameters to improve performance quality in lateral flow immunoassay for detection of PBP2a in methicillin-resistant Staphylococcus aureus (MRSA) |
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| المؤلفون: | M. Amini, M. R. Pourmand, R. Faridi-Majidi, M. Heiat, M. A. Mohammad Nezhady, M. Safari, F. Noorbakhsh, H. Baharifar |
| المصدر: | Journal of Experimental Nanoscience, Vol 15, Iss 1, Pp 266-279 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Chemical technology |
| مصطلحات موضوعية: | lateral flow immunoassay, lfia, gold nanoparticles (aunp), rapid detection, pbp2a, mrsa, Materials of engineering and construction. Mechanics of materials, TA401-492, Chemical technology, TP1-1185 |
| الوصف: | Lateral flow immunoassay (LFIA) is the most widely used platform of the point-of-care (POC) detection. Since the most important factor in deciding the results of this technique is the visual quality of the test, it should be at its optimum. To address this issue, the present study examines optimising critical parameters to improve the quality of LFIA performance. In this regard the penicillin-binding protein 2a (PBP2a) in methicillin-resistant staphylococcus aureus (MRSA) was selected as a bacterial model. The targeted parameters included a) size of gold nanoparticles (AuNP), b) antibody conjugation conditions with AuNP, c) antibody concentrations in the capturing zones, d) the effects of blocking substances and different chemical buffers for pre-treatment of the strips. Visual quality of different manufactured strips revealed that the diameter of 14 ± 2, PH = 9.5 was the optimum for improvement of MRSA detection quality. The optimum concentration of labelling antibody in conjugate pad and concentration of the capturing antibody in the test band were 40 µg/ml and 1.1 µg/μl in PBS buffer (pH 7.4) respectively. In conclusion by finding the critical factors affecting the detection limit of lateral flow and optimising them, the visual quality of LFIA results can be increased. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1745-8080 1745-8099 17458080 |
| Relation: | https://doaj.org/toc/1745-8080; https://doaj.org/toc/1745-8099 |
| DOI: | 10.1080/17458080.2020.1775197 |
| URL الوصول: | https://doaj.org/article/858e5c45f30e4d15bdf9959eb79c5170 |
| رقم الأكسشن: | edsdoj.858e5c45f30e4d15bdf9959eb79c5170 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 17458080 17458099 |
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| DOI: | 10.1080/17458080.2020.1775197 |