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Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss

التفاصيل البيبلوغرافية
العنوان: Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss
المؤلفون: Fredrick E Henry, Ken Sugino, Adam Tozer, Tiago Branco, Scott M Sternson
المصدر: eLife, Vol 4 (2015)
بيانات النشر: eLife Sciences Publications Ltd, 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: hypothalamus, body weight, feeding, arcuate nucleus, RNA-Seq, transcriptomic, Medicine, Science, Biology (General), QH301-705.5
الوصف: Molecular and cellular processes in neurons are critical for sensing and responding to energy deficit states, such as during weight-loss. Agouti related protein (AGRP)-expressing neurons are a key hypothalamic population that is activated during energy deficit and increases appetite and weight-gain. Cell type-specific transcriptomics can be used to identify pathways that counteract weight-loss, and here we report high-quality gene expression profiles of AGRP neurons from well-fed and food-deprived young adult mice. For comparison, we also analyzed Proopiomelanocortin (POMC)-expressing neurons, an intermingled population that suppresses appetite and body weight. We find that AGRP neurons are considerably more sensitive to energy deficit than POMC neurons. Furthermore, we identify cell type-specific pathways involving endoplasmic reticulum-stress, circadian signaling, ion channels, neuropeptides, and receptors. Combined with methods to validate and manipulate these pathways, this resource greatly expands molecular insight into neuronal regulation of body weight, and may be useful for devising therapeutic strategies for obesity and eating disorders.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/09800; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.09800
URL الوصول: https://doaj.org/article/85bf6511747942428dbb33825c644c6a
رقم الأكسشن: edsdoj.85bf6511747942428dbb33825c644c6a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.09800