دورية أكاديمية
Cell painting transfer increases screening hit rate
| العنوان: | Cell painting transfer increases screening hit rate |
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| المؤلفون: | Ethan Cohen, Maxime Corbe, Cláudio A. Franco, Francisca F. Vasconcelos, Franck Perez, Elaine Del Nery, Guillaume Bollot, Auguste Genovesio |
| المصدر: | Biological Imaging, Vol 3 (2023) |
| بيانات النشر: | Cambridge University Press, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Medical technology |
| مصطلحات موضوعية: | high content screening, transfer learning, cell painting, drug discovery, Biology (General), QH301-705.5, Medical technology, R855-855.5 |
| الوصف: | Drug discovery uses high throughput screening to identify compounds that interact with a molecular target or that alter a phenotype favorably. The cautious selection of molecules used for such a screening is instrumental and is tightly related to the hit rate. In this work, we wondered if cell painting, a general-purpose image-based assay, could be used as an efficient proxy for compound selection, thus increasing the success rate of a specific assay. To this end, we considered cell painting images with 30,000 molecules treatments, and selected compounds that produced a visual effect close to the positive control of an assay, by using the Frechet Inception Distance. We then compared the hit rates of such a preselection with what was actually obtained in real screening campaigns. As a result, cell painting would have permitted a significant increase in the success rate and, even for one of the assays, would have allowed to reach 80% of the hits with 10 times fewer compounds to test. We conclude that images of a cell painting assay can be directly used for compound selection prior to screening, and we provide a simple quantitative approach in order to do so. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2633903X 2633-903X |
| Relation: | https://www.cambridge.org/core/product/identifier/S2633903X23000077/type/journal_article; https://doaj.org/toc/2633-903X |
| DOI: | 10.1017/S2633903X23000077 |
| URL الوصول: | https://doaj.org/article/85d3f2e86ce640d79c502ef9afe3ca38 |
| رقم الأكسشن: | edsdoj.85d3f2e86ce640d79c502ef9afe3ca38 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2633903X |
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| DOI: | 10.1017/S2633903X23000077 |