دورية أكاديمية
H3K27me3 Does Not Orchestrate the Expression of Lineage-Specific Markers in hESC-Derived Hepatocytes In Vitro
| العنوان: | H3K27me3 Does Not Orchestrate the Expression of Lineage-Specific Markers in hESC-Derived Hepatocytes In Vitro |
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| المؤلفون: | Jolien Vanhove, Mariaelena Pistoni, Marc Welters, Kristel Eggermont, Veerle Vanslembrouck, Nicky Helsen, Ruben Boon, Mustapha Najimi, Etienne Sokal, Philippe Collas, J. Willem Voncken, Catherine M. Verfaillie |
| المصدر: | Stem Cell Reports, Vol 7, Iss 2, Pp 192-206 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Although pluripotent stem cells can be differentiated into the hepatocyte lineages, such cells retain an immature phenotype. As the chromatin state of regulatory regions controls spatiotemporal gene expression during development, we evaluated changes in epigenetic histone marks in lineage-specific genes throughout in vitro hepatocyte differentiation from human embryonic stem cells (hESCs). Active acetylation and methylation marks at promoters and enhancers correlated with progressive changes in gene expression. However, repression-associated H3K27me3 marks at these control regions showed an inverse correlation with gene repression during transition from hepatic endoderm to a hepatocyte-like state. Inhibitor of Enhancer of Zeste Homolog 2 (EZH2) reduced H3K27me3 decoration but did not improve hepatocyte maturation. Thus, H3K27me3 at regulatory regions does not regulate transcription and appears dispensable for hepatocyte lineage differentiation of hESCs in vitro. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-6711 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213671116301059; https://doaj.org/toc/2213-6711 |
| DOI: | 10.1016/j.stemcr.2016.06.013 |
| URL الوصول: | https://doaj.org/article/c861824b218e473a89560f975a0b24c8 |
| رقم الأكسشن: | edsdoj.861824b218e473a89560f975a0b24c8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2016.06.013 |