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Establishment of a Practical Sperm Cryopreservation Pathway for the Axolotl (Ambystoma mexicanum): A Community-Level Approach to Germplasm Repository Development

التفاصيل البيبلوغرافية
العنوان: Establishment of a Practical Sperm Cryopreservation Pathway for the Axolotl (Ambystoma mexicanum): A Community-Level Approach to Germplasm Repository Development
المؤلفون: Nicholas Coxe, Yue Liu, Lucía Arregui, Rose Upton, Sarah Bodenstein, Steven Randal Voss, Maria T. Gutierrez-Wing, Terrence R. Tiersch
المصدر: Animals, Vol 14, Iss 2, p 206 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Veterinary medicine
LCC:Zoology
مصطلحات موضوعية: axolotl, Ambystoma, sperm, cryopreservation, pathway, process map, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: The axolotl (Ambystoma mexicanum) draws great attention around the world for its importance as a biomedical research model, but housing and maintaining live animals is increasingly expensive and risky as new transgenic lines are developed. The goal of this work was to develop an initial practical pathway for sperm cryopreservation to support germplasm repository development. The present study assembled a pathway through the investigation of axolotl sperm collection by stripping, refrigerated storage in various osmotic pressures, cryopreservation in various cryoprotectants, and in vitro fertilization using thawed sperm. By the stripping of males, 25–800 µL of sperm fluid was collected at concentrations of 1.6 × 106 to 8.9 × 107 sperm/mL. Sperm remained motile for 5 d in Hanks’ Balanced Salt Solution (HBSS) at osmolalities of 100–600 mOsm/kg. Sperm cryopreserved in 0.25 mL French straws at 20 °C/min in a final concentration of 5% DMFA plus 200 mM trehalose and thawed at 25 °C for 15 s resulted in 52 ± 12% total post-thaw motility. In six in vitro fertilization trials, 20% of eggs tested with thawed sperm continued to develop to stage 7–8 after 24 h, and a third of those embryos (58) hatched. This work is the first report of successful production of axolotl offspring with cryopreserved sperm, providing a general framework for pathway development to establish Ambystoma germplasm repositories for future research and applications.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-2615
Relation: https://www.mdpi.com/2076-2615/14/2/206; https://doaj.org/toc/2076-2615
DOI: 10.3390/ani14020206
URL الوصول: https://doaj.org/article/a862d83300114ce8b51fd14afadaf13f
رقم الأكسشن: edsdoj.862d83300114ce8b51fd14afadaf13f
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20762615
DOI:10.3390/ani14020206