دورية أكاديمية
Identification of a dominant murine T-cell epitope in recombinant protein P29 from Echinococcus granulosus
| العنوان: | Identification of a dominant murine T-cell epitope in recombinant protein P29 from Echinococcus granulosus |
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| المؤلفون: | Lv Yongxue, Zhu Yazhou, Chang Liangliang, Yang Jihui, Zhao Yinqi, Zhao Jiaqing, Wang Yana, Zhu Mingxing, Wu Changyou, Zhao Wei |
| المصدر: | Acta Biochimica et Biophysica Sinica, Vol 54, Pp 482-493 (2022) |
| بيانات النشر: | China Science Publishing & Media Ltd., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biochemistry LCC:Genetics |
| مصطلحات موضوعية: | Echinococcus granulosus, rEg.P29, T-cell epitope, Biochemistry, QD415-436, Genetics, QH426-470 |
| الوصف: | Echinococcus granulosus causes echinococcosis, an important zoonotic disease worldwide and a major public health issue. Vaccination is an economical and practical approach for controlling E. granulosus. We have previously revealed that a recombinant protein P29 (rEg.P29) is a good vaccine candidate against E. granulosus. However, T cell immunogenic epitopes have not been identified. In the present study, we use rEg.P29-immunized mice as models to screen immunogenic epitopes for the construction of a novel multi-epitope vaccine. We search for immunodominant epitopes from an overlapping peptide library to screen the peptides of rEg.P29. Our results confirm that rEg.P29 immunization in mice elicits the activation of T cells and induces cellular immune responses. Further analyses show that a T cell epitope within amino acids 86–100 of rEg.P29 elicits significant antigen-specific IFN-γ production in CD4+ and CD8+ T cells and promotes specific T-cell activation and proliferation. Collectively, these results provide a reference for the construction of a novel vaccine against broad E. granulosus genotypes based on epitopes of rEg.P29. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1672-9145 |
| Relation: | https://doaj.org/toc/1672-9145 |
| DOI: | 10.3724/abbs.2022036 |
| URL الوصول: | https://doaj.org/article/86814030b0424f30904b613ebd16ad30 |
| رقم الأكسشن: | edsdoj.86814030b0424f30904b613ebd16ad30 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16729145 |
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| DOI: | 10.3724/abbs.2022036 |