دورية أكاديمية
MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
| العنوان: | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
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| المؤلفون: | Hai-Bin Liang, Yang Cao, Qiang Ma, Yi-Jun Shu, Zheng Wang, Fei Zhang, Yuan-Yuan Ye, Huai-Feng Li, Shan-Shan Xiang, Xiao-Ling Song, Yi Xu, Yi-Chi Zhang, Run-Fa Bao, Rui-Yan Yuan, Yi-Jian Zhang, Yun-Ping Hu, Lin Jiang, Mao-Lan Li, Xu-An Wang, Xiang-Song Wu, Wen-Guang Wu, Shuai Zhao, Yong Fand, Xiao-Peng Cui, Yun-Shu Lu, Jian Zhou, Lei Zheng, Wei Gong, Ying-Bin Liu |
| المصدر: | Cellular Physiology and Biochemistry, Vol 41, Iss 5, Pp 2117-2131 (2017) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Physiology LCC:Biochemistry |
| مصطلحات موضوعية: | Cell cycle, S phase, MYBL2, Gallbladder cancer, Cell proliferation, G2/M phase, Physiology, QP1-981, Biochemistry, QD415-436 |
| الوصف: | Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1015-8987 1421-9778 |
| Relation: | http://www.karger.com/Article/FullText/475454; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000475454 |
| URL الوصول: | https://doaj.org/article/86f84e46e1bf4cf5886bfd7baae39170 |
| رقم الأكسشن: | edsdoj.86f84e46e1bf4cf5886bfd7baae39170 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10158987 14219778 |
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| DOI: | 10.1159/000475454 |