دورية أكاديمية
Biomimetic cultivation of atrial tissue slices as novel platform for in-vitro atrial arrhythmia studies
| العنوان: | Biomimetic cultivation of atrial tissue slices as novel platform for in-vitro atrial arrhythmia studies |
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| المؤلفون: | Jorik H. Amesz, Natasja M. S. de Groot, Sanne J. J. Langmuur, Hamid el Azzouzi, Vera P. C. Tiggeloven, Manuela M. M. M. van Rooij, P. Knops, Ad J. J. C. Bogers, Yannick J. H. J. Taverne |
| المصدر: | Scientific Reports, Vol 13, Iss 1, Pp 1-8 (2023) |
| بيانات النشر: | Nature Portfolio, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Living myocardial slices (LMS) are beating sections of intact human myocardium that maintain 3D microarchitecture and multicellularity, thereby overcoming most limitations of conventional myocardial cell cultures. We introduce a novel method to produce LMS from human atria and apply pacing modalities to bridge the gap between in-vitro and in-vivo atrial arrhythmia studies. Human atrial biopsies from 15 patients undergoing cardiac surgery were dissected to tissue blocks of ~ 1 cm2 and cut to 300 µm thin LMS with a precision-cutting vibratome. LMS were placed in a biomimetic cultivation chamber, filled with standard cell culture medium, under diastolic preload (1 mN) and continuous electrical stimulation (1000 ms cycle length (CL)), resulting in 68 beating LMS. Atrial LMS refractory period was determined at 192 ± 26 ms. Fixed rate pacing with a CL of 333 ms was applied as atrial tachyarrhythmia (AT) model. This novel state-of-the-art platform for AT research can be used to investigate arrhythmia mechanisms and test novel therapies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-023-30688-8 |
| URL الوصول: | https://doaj.org/article/871f95f2a5d941a19aa9ffb411fa7460 |
| رقم الأكسشن: | edsdoj.871f95f2a5d941a19aa9ffb411fa7460 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-023-30688-8 |