دورية أكاديمية
A zebrafish model to elucidate the impact of host genes on the microbiota
| العنوان: | A zebrafish model to elucidate the impact of host genes on the microbiota |
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| المؤلفون: | Eiríkur Andri Thormar, Jacob Agerbo Rasmussen, Heidi Mathiessen, Moonika Haahr Marana, Cecilie Grønlund Clausen, Martin Hansen, Miyako Kodama, Louise vonGersdorff Jørgensen, Morten Tønsberg Limborg |
| المصدر: | Environmental DNA, Vol 6, Iss 1, Pp n/a-n/a (2024) |
| بيانات النشر: | Wiley, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Environmental sciences LCC:Microbial ecology |
| مصطلحات موضوعية: | genetic engineering, hologenomics, host effects on microbiota, host–microbiota interactions, metabarcoding, untargeted metabolomics, Environmental sciences, GE1-350, Microbial ecology, QR100-130 |
| الوصف: | Abstract Every host species and organism provides a unique environmental niche contributing to the overall diversity of microbial ecosystems from the intestine of an animal to the oceans and forests of our planet. The study of host–microbiota interactions has long focused on the well‐established effects the microbiota has on its host. In contrast, little focus has been allocated to the role of the host in these intricate interactions. However, understanding the role of the host may well be an essential key to understanding the complexity of the relationship between the host and its microbiota. In this study, we present a model in which the effects of host genes on the microbiota can be elucidated and how such genetic effects may shape host‐associated microbiota. We demonstrate a hologenomic approach implementing the CRISPR/Cas system in the zebrafish model to combine the effects of a host gene with 16S metabarcoding and metabolomics data. We show that knocking out the gene coding for the rate‐limiting enzyme in melanogenesis, tyrosinase (tyr), correlates with changes in the intestinal microbiota of zebrafish and differences in the abundance of specific metabolites illustrating the value of our model for studying the impact of host genes on the composition and function of the intestinal microbiota. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2637-4943 |
| Relation: | https://doaj.org/toc/2637-4943 |
| DOI: | 10.1002/edn3.513 |
| URL الوصول: | https://doaj.org/article/87210286046b4b1486cbb63cd1526f5a |
| رقم الأكسشن: | edsdoj.87210286046b4b1486cbb63cd1526f5a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26374943 |
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| DOI: | 10.1002/edn3.513 |