The current study aimed to analyze the stabilization of sunflower oil using white mustard (Brassica hirta) leaf extracts as well as their antioxidant potential and cytotoxicity. Selected plant leaves were macerated in different solvents such as n-hexane, chloroform, ethyl acetate, n-butanol, and methanol. The total flavonoid contents, total phenolic contents, the antioxidant activity by DPPH assay, and the inhibition of linoleic acid oxidation were also examined. Moreover, the stabilization of sunflower oil was evaluated by calculating the conjugated dienes, conjugated trienes, peroxide value, p-ansidine, and free fatty acid values. The cytotoxicity study in terms of the hemolytic activity of B. hirta leaf extract was evaluated and the maximum percentage of lysis was found to be safe (less than 5%). The results of the present study revealed that among others, methanolic white mustard (B. hirta) leaf extract possessed higher antioxidant potential and might be safely employed for the stabilization of vegetable oils.
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