دورية أكاديمية
Comparison of the mechanisms of estrogen disrupting effects between triphenyl phosphate (TPhP) and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP)
| العنوان: | Comparison of the mechanisms of estrogen disrupting effects between triphenyl phosphate (TPhP) and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) |
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| المؤلفون: | Xiaoya Ji, Na Li, Mei Ma, Xinyan Li, Kongrui Zhu, Kaifeng Rao, Zijian Wang, Jingfeng Wang, Yanjun Fang |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 229, Iss , Pp 113069- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | Triphenyl phosphate (TPhP), Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), Estrogen disrupting effects, Estrogen receptor α (ERα), G protein-coupled estrogen receptor (GPER), Estrogen biosynthesis, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | As the typical aryl-organophosphate flame retardants (OPFRs), triphenyl phosphate (TPhP) and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) were reported to be estrogen disruptors. However, estrogen receptor α (ERα) binding experiments could not explain their biological effects. In this study, their action on ERα, G protein-coupled estrogen receptor (GPER) and the synthesis of 17β-estradiol (E2) were investigated using in vitro assays and molecular docking. The results showed that TPhP acted as an ERα agonist and recruited steroid receptor co-activator 1 (SRC1) and 3 (SRC3), which was found for the first time. Unlike TPhP, TDCIPP acted as an ERα antagonist. However, both TPhP and TDCIPP activated the estrogen pathway by GPER in SKBR3 cells which were lack of ERα. Although molecular docking results revealed that both TPhP and TDCIPP could dock into ERα and GPER, their substituent groups and combination mode might affect the receptor activation. In addition, by using estrogen biosynthesis assay in H295R cells, both of TPhP and TDCIPP were found to promote E2 synthesis and E2/T ratio involving their different alteration on levels of progesterone, testosterone and estrone, and expression of various key genes. Our data proposed estrogen-disrupting mechanism frameworks of TPhP and TDCIPP. Moreover, our results will contribute to future construction of adverse outcome pathway (AOP) framework of endocrine disruptors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651321011817; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2021.113069 |
| URL الوصول: | https://doaj.org/article/d87cf5a7eb2f40d2b8be89c6f7affa55 |
| رقم الأكسشن: | edsdoj.87cf5a7eb2f40d2b8be89c6f7affa55 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 |
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| DOI: | 10.1016/j.ecoenv.2021.113069 |