دورية أكاديمية
SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent manner
| العنوان: | SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent manner |
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| المؤلفون: | Yun-Qi Zhou, Ke Wang, Xue-Yan Wang, Hong-Yong Cui, Yongxiang Zhao, Ping Zhu, Zhi-Nan Chen |
| المصدر: | Emerging Microbes and Infections, Vol 11, Iss 1, Pp 1135-1144 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | SARS-CoV-2, CD147, endocytosis, Arf6, spike protein, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants is threatening public health around the world. Endocytosis functions as an important way for viral infection, and SARS-CoV-2 bears no exception. However, the specific endocytic mechanism of SARS-CoV-2 remains unknown. In this study, we used endocytic inhibitors to evaluate the role of different endocytic routes in SARS-CoV-2 pseudovirus infection and found that the viral infection was associated with caveolar/lipid raft- and cytoskeleton-mediated endocytosis, but independent of the clathrin-mediated endocytosis and macropinocytosis. Meanwhile, the knockdown of CD147 and Rab5a in Vero E6 and Huh-7 cells inhibited SARS-CoV-2 pseudovirus infection, and the co-localization of spike protein, CD147, and Rab5a was observed in pseudovirus-infected Vero E6 cells, which was weakened by CD147 silencing, illustrating that SARS-CoV-2 pseudovirus entered the host cells via CD147-mediated endocytosis. Additionally, Arf6 silencing markedly inhibited pseudovirus infection in Vero E6 and Huh-7 cells, while little change was observed in CD147 knockout-Vero E6 cells. This finding indicated Arf6-mediated CD147 trafficking plays a vital role in SARS-CoV-2 entry. Taken together, our findings provide new insights into the CD147-Arf6 axis in mediating SARS-CoV-2 pseudovirus entry into the host cells, and further suggest that blockade of this pathway seems to be a feasible approach to prevent the SARS-CoV-2 infection clinically. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2022.2059403 |
| URL الوصول: | https://doaj.org/article/87dade94fdb145869c4fedb680946111 |
| رقم الأكسشن: | edsdoj.87dade94fdb145869c4fedb680946111 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2022.2059403 |