دورية أكاديمية
Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations
| العنوان: | Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations |
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| المؤلفون: | Emma Ispasanie, Francesca Micoli, Araceli Lamelas, Dominique Keller, Francesco Berti, Riccardo De Ricco, Roberta Di Benedetto, Simona Rondini, Gerd Pluschke |
| المصدر: | Virulence, Vol 9, Iss 1, Pp 1138-1149 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Neisseria meningitidis, capsule synthesis locus, genomic diversity, herd immunity, virulence, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Whole genome sequencing analysis of 100 Neisseria meningitidis serogroup A isolates has revealed that the csaABCD-ctrABCD-ctrEF capsule polysaccharide synthesis locus represents a spontaneous point mutation hotspot. Structural and functional properties of the capsule of 11 carriage and two disease isolates with non-synonymous point mutations or stop codons in capsule synthesis genes were analyzed for their capsular polysaccharide expression, recognition by antibodies and sensitivity to bactericidal killing. Eight of eleven carriage isolates presenting capsule locus mutations expressed no or reduced amounts of capsule. One isolate with a stop codon in the O-acetyltransferase gene expressed non-O-acetylated polysaccharide, and was not recognized by anti-capsule antibodies. Capsule and O-acetylation deficient mutants were resistant to complement deposition and killing mediated by anti-capsular antibodies, but not by anti-lipopolysaccharide antibodies. Two capsule polymerase mutants, one carriage and one case isolate, showed capsule over-expression and increased resistance against bactericidal activity of both capsule- and lipopolysaccharide-specific antibodies. Meningococci have developed multiple strategies for changing capsule expression and structure, which is relevant both for colonization and virulence. Here we show that point mutations in the capsule synthesis genes substantially contribute to the repertoire of genetic mechanisms in natural populations leading to variability in capsule expression. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2150-5594 2150-5608 21505594 |
| Relation: | https://doaj.org/toc/2150-5594; https://doaj.org/toc/2150-5608 |
| DOI: | 10.1080/21505594.2018.1467710 |
| URL الوصول: | https://doaj.org/article/a87de0aba4ed4a468df8f59402df92d0 |
| رقم الأكسشن: | edsdoj.87de0aba4ed4a468df8f59402df92d0 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 21505594 21505608 |
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| DOI: | 10.1080/21505594.2018.1467710 |