دورية أكاديمية

Automated Imaging to Evaluate the Exogenous Gibberellin (Ga3) Impact on Seedlings from Salt-Stressed Lettuce Seeds

التفاصيل البيبلوغرافية
العنوان: Automated Imaging to Evaluate the Exogenous Gibberellin (Ga3) Impact on Seedlings from Salt-Stressed Lettuce Seeds
المؤلفون: Mark Iradukunda, Marc W. van Iersel, Lynne Seymour, Guoyu Lu, Rhuanito Soranz Ferrarezi
المصدر: Sensors, Vol 24, Iss 13, p 4228 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Chemical technology
مصطلحات موضوعية: Lactuca sativa, canopy size, crop modeling, germination, image analysis, salinity, Chemical technology, TP1-1185
الوصف: Salinity stress is a common challenge in plant growth, impacting seed quality, germination, and general plant health. Sodium chloride (NaCl) ions disrupt membranes, causing ion leakage and reducing seed viability. Gibberellic acid (GA3) treatments have been found to promote germination and mitigate salinity stress on germination and plant growth. ‘Bauer’ and ‘Muir’ lettuce (Lactuca sativa) seeds were soaked in distilled water (control), 100 mM NaCl, 100 mM NaCl + 50 mg/L GA3, and 100 mM NaCl + 150 mg/L GA3 in Petri dishes and kept in a dark growth chamber at 25 °C for 24 h. After germination, seedlings were monitored using embedded cameras, capturing red, green, and blue (RGB) images from seeding to final harvest. Despite consistent germination rates, ‘Bauer’ seeds treated with NaCl showed reduced germination. Surprisingly, the ‘Muir’ cultivar’s final dry weight differed across treatments, with the NaCl and high GA3 concentration combination yielding the poorest results (p < 0.05). This study highlights the efficacy of GA3 applications in improving germination rates. However, at elevated concentrations, it induced excessive hypocotyl elongation and pale seedlings, posing challenges for two-dimensional imaging. Nonetheless, a sigmoidal regression model using projected canopy size accurately predicted dry weight across growth stages and cultivars, emphasizing its reliability despite treatment variations (R2 = 0.96, RMSE = 0.11, p < 0.001).
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 24134228
1424-8220
Relation: https://www.mdpi.com/1424-8220/24/13/4228; https://doaj.org/toc/1424-8220
DOI: 10.3390/s24134228
URL الوصول: https://doaj.org/article/a88523b7692f412eaf279fd5403e56e8
رقم الأكسشن: edsdoj.88523b7692f412eaf279fd5403e56e8
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:24134228
14248220
DOI:10.3390/s24134228