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Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution

التفاصيل البيبلوغرافية
العنوان: Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution
المؤلفون: Azul Zorzoli, Alasdair MacLean, Scott Nicholson, Alison Daniels, Stephen Hughes, Susan Bennet-Slater, Christine Tait-Burkard, Noha El Sakka, Rory Gunson, Kate Templeton
المصدر: BioTechniques, Vol 76, Iss 7, Pp 295-298 (2024)
بيانات النشر: Taylor & Francis Group, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: cycle threshold (Ct), SARS-CoV-2, Tergitol 15-S-9, Triton X-100, viral load, Biology (General), QH301-705.5
الوصف: In 2019, the European Union banned Triton X-100, a detergent widely used in laboratory diagnostics, including the Viral PCR Sample Solution (VPSS), and urged manufacturers to find environmentally sustainable alternatives. Tergitol 15-S-9 (VPSS2) has been proposed as an alternative surfactant. This multicenter study evaluated the effectiveness of VPSS2, a Tergitol-based viral solution, as a replacement for VPSS. Our results show the equivalent performance of VPSS2 to VPSS for nucleic acid extraction and viral stability over time at different temperatures. The new VPSS formulation was also tested against external quality assurance panels and clinical samples. The results of this work support adopting this modified viral PCR sample solution to replace Triton X-100-containing viral transport solutions.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 07366205
1940-9818
0736-6205
Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818
DOI: 10.1080/07366205.2024.2342172
URL الوصول: https://doaj.org/article/894f8e032c58490c9961699e127ca8df
رقم الأكسشن: edsdoj.894f8e032c58490c9961699e127ca8df
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:07366205
19409818
DOI:10.1080/07366205.2024.2342172