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Elements modulating the prion species barrier and its passage consequences.

التفاصيل البيبلوغرافية
العنوان: Elements modulating the prion species barrier and its passage consequences.
المؤلفون: Juan-Maria Torres, Juan-Carlos Espinosa, Patricia Aguilar-Calvo, María-Eugenia Herva, Aroa Relaño-Ginés, Ana Villa-Diaz, Mónica Morales, Beatriz Parra, Elia Alamillo, Alejandro Brun, Joaquín Castilla, Susana Molina, Steve A C Hawkins, Olivier Andreoletti
المصدر: PLoS ONE, Vol 9, Iss 3, p e89722 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The specific characteristics of Transmissible Spongiform Encephalopathy (TSE) strains may be altered during passage across a species barrier. In this study we investigated the biochemical and biological characteristics of Bovine Spongiform Encephalopathy (BSE) after transmission in both natural host species (cattle, sheep, pigs and mice) and in transgenic mice overexpressing the corresponding cellular prion protein (PrPC) in comparison with other non-BSE related prions from the same species. After these passages, most features of the BSE agent remained unchanged. BSE-derived agents only showed slight modifications in the biochemical properties of the accumulated PrPSc, which were demonstrated to be reversible upon re-inoculation into transgenic mice expressing bovine-PrPC. Transmission experiments in transgenic mice expressing bovine, porcine or human-PrP revealed that all BSE-derived agents were transmitted with no or a weak transmission barrier. In contrast, a high species barrier was observed for the non-BSE related prions that harboured an identical PrP amino acid sequence, supporting the theory that the prion transmission barrier is modulated by strain properties (presumably conformation-dependent) rather than by PrP amino acid sequence differences between host and donor. As identical results were observed with prions propagated either in natural hosts or in transgenic mouse models, we postulate that the species barrier and its passage consequences are uniquely governed by the host PrPC sequence and not influenced by other host genetic factors. The results presented herein reinforce the idea that the BSE agent is highly promiscuous, infecting other species, maintaining its properties in the new species, and even increasing its capabilities to jump to other species including humans. These data are essential for the development of an accurate risk assessment for BSE.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3946430?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0089722
URL الوصول: https://doaj.org/article/c89528df259e4d1b9e9b89ba0f1bf709
رقم الأكسشن: edsdoj.89528df259e4d1b9e9b89ba0f1bf709
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0089722